Zeocin™ | Selection antibiotic(IC-1610)
BackGround
Zeocin™ is a formulation of phleomycin D1, a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990. Zeocin™ causes cell death by intercalating into DNA and cleaving it.
The action of Zeocin™ is effective on most aerobic cells. Therefore, Zeocin™ is a popular and effective antibiotic for the selection of vectors bearing the Sh ble gene in a variety of cells types (bacteria, eukaryotic microorganisms, plant and animal cells).
Resistance to Zeocin™ is conferred by the Sh ble gene product from Streptoalloteichus hindustanus, which inactivates Zeocin™ upon binding to the antibiotic [1-3].
Sh ble is a small 370bp-sized gene and is carried in a number of InvivoGen¡¯s vectors.
Typically, mammalian cells are sensitive to Zeocin™ concentrations of 50-400 ¦Ìg/ml, and bacteria to 25 ¦Ìg/ml.
For Research Use Only. Not for use in diagnostic procedures.
References
1. Drocourt D. et al., 1990. Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance. Nucl. Acids. Res. 18: 4009.
2. Gatignol A. et al., 1988. FEBS Letters. 230: 171-5.
3. Dumas P. et al., 1994. Embo J. 242 (5) 595-601.
Specifications
Product concentration: 100 mg/ml in solution
CAS number: 11006-33-0
Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Endotoxin level: < 1 EU/mg
Physicochemical characterization: HPLC, pH, appearance
Cell-culture tested: potency validated in Zeocin™-sensitive and Zeocin™-resistant mammalian cell lines
Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in Zeocin™-resistant cells
Contents
Zeocin™ is supplied as a sterile filtered blue solution at 100 mg/ml in HEPES buffer.
This product is available in three pack sizes:
- IC-1610-A: 1 ml
- IC-1610-B: 5 x 1ml (500mg)
- IC-1610-C: 10 x 1ml (1g)
Zeocin™ is also supplied as a blue powder:
- IC-1611-A: 1 g
- IC-1611-B: 5 g
Zeocin™ is shipped at room temperature.
Upon receipt it should be stored at 4¡ãC or -20¡ãC.
Zeocin™ is a harmful compound. Refer to safety data sheet for handling instructions.
Zeocin™ is a trademark of Invivo.
