Lipopolysaccharides/LPS,297-473-0

Cat.No.:

IC-0225458

Product Information

Biological Activity

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).                                                                                      
LPS is a major constituent of the cell wall of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. LPS also acts as a specific mitogen for bone marrow derived B lymphocytes from mice, rabbits, chickens, cows, hamsters, and humans.                                                                                                                    
Lipopolysaccharides are supplied as lyophilized, ¦Ã-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species

Mouse

Rat

Rabbit

Guinea pig

Hamster

Dog

Weight (kg)

0.02

0.15

1.8

0.4

0.08

10

Body Surface Area (m2)

0.007

0.025

0.15

0.05

0.02

0.5

Km factor

3

6

12

8

5

20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by  (Animal B Km / Animal A Km)
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Order Information

From Escherichia coli 055:B5

Quantity

Price($£©

Price(€)

Price(£¤/CNY£©

Price(£¤/JYP£©

10mg

$80.00

€96.00

£¤800.00

¥15,920.00

50mg

$300.00

€360.00

£¤3,000.00

¥59,700.00

100mg

$550.00

€660.00

£¤5,500.00

¥109,450.00

Free Delivery on orders over $350.00.

Chemical Information

Appearance (Form)

Lyophilized Powder

Application(s)

Cell culture | Mammalian: suitable

CAS Number

297-473-0

Purity

>99.00%

Solubility

Soluble in water (10 mg/ml) or cell culture medium (1 mg/ml)

Storage

at 2-8¡ãC

Reference

[1]. Kabanov DS, et al. Structural analysis of lipopolysaccharides from Gram-negative bacteria. Biochemistry (Mosc). 2010 Apr;75(4):383-404.

[2]. Heinrichs DE, et al. Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica. Mol Microbiol. 1998 Oct;30(2):221-32.

[3]. Cai KC, et al. Age and sex differences in immune response following LPS treatment in mice. Brain Behav Immun. 2016 Nov;58:327-337.

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