Recombinant Human Interleukin-10(IL-10)
Cat.No.:CYTH0004
Human Interleukin-10
Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of ¦Á- helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 k Da noncovalently associated homodimer. The IL-10 dimer binds to two IL-10 R¦Á/IL- 10R1 chains, resulting in recruitment of two IL-10 R¦Â/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL-10R¦Â does not bind IL-10 by itself but is required for signal transduction. IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.
Source: Escherichia coli.
Molecular Weight: Approximately 18.6 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids.
Size:2¦Ìg/10¦Ìg/1000¦Ìg
AA Sequence:SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN
Purity:> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity:Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0x106 IU/mg.
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2 ¦Ìm filtered concentrated solution in PBS, pH 7.4.
Endotoxin:Less than 1 EU/¦Ìg of rHuIL-10 as determined by LAL method.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Ü -20¡æ. Further dilutions should be made in appropriate buffered solutions.
Shipping:The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
l-12 months from date of receipt, -20¡æ to -70¡æ as supplied.
l-1 month, 2¡æ to 8¡æ under sterile conditions after reconstitution.
l-3 months, -20 to -70¡æ under sterile conditions after reconstitution.
Usage:This material is offered by InCellGene for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
