Metformin hydrochloride,1115-70-4

Cat.No.:

IC-0128555

Product Information                                                               

Biological Activity

Metformin hydrochloride (1,1-Dimethylbiguanide hydrochloride) inhibits the mitochondrial respiratory chain in the liver, leading to activation of AMPK, enhancing insulin sensitivity for type 2 diabetes research. Metformin hydrochloride triggers autophagy[1]. Metformin hydrochloride (1,1-Dimethylbiguanide hydrochloride) inhibits proliferation of ESCs in a concentration-dependent manner. The IC50 is 2.45 mM for A-ESCs and 7.87 mM for N-ESCs. Metformin shows pronounced effects on activation of AMPK signaling in A-ESCs from secretory phase than in cells from proliferative phase[2].
Metformin hydrochloride (0-500 ¦ÌM) decreases glycogen synthesis in a dose-dependent manner with an IC50 value of 196.5 ¦ÌM in cultured rat hepatocytes[3].
Metformin hydrochloride shows cell viability and cytotoxic effects on PC-3 cells with IC50 of 5 mM[4].Metformin hydrochloride (1,1-Dimethylbiguanide hydrochloride; 100 mg/kg, p.o.) alone, and metformin (25, 50, 100 mg/kg) with NSC 37745 groups attenuates myocyte necrosis through histopathological analysis[1].

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species

Mouse

Rat

Rabbit

Guinea pig

Hamster

Dog

Weight (kg)

0.02

0.15

1.8

0.4

0.08

10

Body Surface Area (m2)

0.007

0.025

0.15

0.05

0.02

0.5

Km factor

3

6

12

8

5

20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by  (Animal B Km / Animal A Km)
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Order Information

Size

Price($£©

Price(€)

Price(£¤/CNY£©

Price(£¤/JYP£©

1g

$80.00

€ 96.00

£¤800.00

£¤15,920.00

5g

$120.00

€ 144.00

£¤1,200.00

£¤23,880.00

25g

$199.00

€ 238.80

£¤1,990.00

£¤39,601.00

Free Delivery on orders over $350.00.

Chemical Information

Molecular Weight

165.62

Formula

C₄H₁₂ClN₅

CAS Number

1115-70-4

Purity

>98%

Solubility

H2O : 250 mg/mL (1509.48 mM; Need ultrasonic),DMSO : ¡Ý 1.7 mg/mL (10.26 mM)

Storage

at -20¡ãC for 3years,at 2-8¡ãC for 1 year

Reference

[1]. Soraya H, et al. Acute treatment with metformin improves cardiac function following NSC 37745 induced myocardial infarction in rats. Pharmacol Rep. 2012;64(6):1476-84.

[2]. Xue J, et al. Metformin inhibits growth of eutopic stromal cells from adenomyotic endometrium via AMPK activation and subsequent inhibition of AKT phosphorylation: a possible role in the treatment of adenomyosis. Reproduction. 2013 Aug 21;146(4):397-406.                                                                                                                                                                                                                          [3]. Otto M, et al. Metformin inhibits glycogen synthesis and gluconeogenesis in cultured rat hepatocytes. Diabetes Obes Metab. 2003 May;5(3):189-94.
[4]. Avci CB, et al. Therapeutic potential of an anti-diabetic drug, metformin: alteration of miRNA expression in prostate cancer cells. Asian Pac J Cancer Prev. 2013;14(2):765-8.

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