DOTA-NHS-ester,				170908-81-3
			Cat.No.:	IC-0172809
Product Information
Biological Activity
DOTA-NHS-ester is a linker for affibody molecules and is applied in small animals PET, SPECT, and CT. DOTA-NHS-ester can be used to label radiotherapeutic agents or imaging probes for the detection of tumors[1]. DOTA-NHS-ester can be used to modify human serum albumin (HSA) to produce DOTA-HSA. And DOTA-HSA is furtherly modified by Sulfo-SMCC to obtain DOTA-HSA-SMCC. DOTA-HSA-SMCC is conjugated to ZHER2:342 and the final product is DOTA-HSA-ZHER2:342[1]. In a cell uptake assay, DOTA-HSA-ZHER2:342 is labeled by 64Cu, 64Cu-DOTA-HSA-ZHER2:342 (0.5-2 hours) slowly accumulates in the SKOV3 cells and reaches 0.71% of the applied activity at 0.5 h and the uptake increased to 1.58% at 2 h[1]. In a microPET images of a mouse bearing SKOV3 tumor, 64Cu-DOTA-HSA-ZHER2:342 is injected to the mouse tail. microPET images of a mouse bearing SKOV3 tumor at 1, 4, 24 and 48 h after tail vein injection.The SKOV3 tumor is visible with a low tumor-to-background contrast at 1 h post-injection (p.i.), but with a very good tumor-to-background contrast at 4 and 24 h p.i.Quantification analysis reveals that the SKOV3 tumor uptake values increases with time and are found to be 5.63%, 9.98%, 14.34% and 14.12% ID/g at 1, 4, 24, and 48 h, respectively[1].
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species		Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)		0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)		0.007	0.025	0.15	0.05	0.02	0.5
Km factor		3	6	12	8	5	20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  (Animal B Km / Animal A Km) For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
Order Information
				Quantity	Price($)	Price($)	Availability
				5mg	$300.00	€330.00	In stock
				10mg	$400.00	€440.00	In stock
				50mg	$900.00	€990.00	In stock
				Free Delivery on orders over $350.00.
Chemical Information
Molecular Weight		501.49
Formula		C20H31N5O10
CAS Number		170908-81-3
Purity		>98%
Solubility		DMSO: 50 mg/mL (99.70 mM)
Storage		at -20¡ãC
Reference
[1]. Hoppmann S, et al. Radiolabeled affibody-albumin bioconjugates for HER2-positive cancer targeting.Bioconjug Chem. 2011 Mar 16;22(3):413-2
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