Cat.No.:

IC-7902

Product Information

Protease Inhibitor Cocktail,EDTA Free ,100X are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction.

• Convenient¡ªthe refrigerator-stable, 100X liquid format is more effective and easier to use than individual inhibitors or other formats; just pipette exactly the amount you need
• No proprietary ingredients¡ªfully disclosed formulation contains optimized concentrations of Nine broad-spectrum protease inhibitors AEBSF£¬Aprotinin£¬Bestatin£¬E-64£¬ Leupeptin£¬Pepstatin A£¬Phosphoramidon£¬Benzamidine HCl£¬1,10-phenanthroline stabilized in high-quality dimethylsulfoxide (DMSO).
• Multiple package sizes¡ªchoose among several package sizes, including single-use microtubes that keep protease inhibitors fresh and reduce the risk of reagent contamination.
• Regular formulation¡ªincludes a separate vial of EDTA solution for optional metalloprotease inhibition.
• Lysis buffer compatible¡ªuse with Thermo Scientific Pierce Cell Lysis Buffers or nearly any other commercial or homemade detergent-based lysis reagent.

Applications

• Protection of intact, active cellular proteins from degradation by endogenous proteases
• Inhibition of protease activity from lysates produced by a variety of methods including  RIPA Lysis Reagents, other commercially formulated cell or tissue lysis products, sonication, French press, blender homogenization or repetitive freeze/thaw cycling
• Screening of extracts for proteolytic activity
• The study of proteolysis in the regulation of cellular processes

Usage Information

• Add 10 ¦ÌL of concentrated cocktail per 1 mL of lysis buffer to ensure complete protection of the resulting protein extract.
• The single-use format is ideal for small-scale cell lysis experiments, where each 100 ¦ÌL vial provides just what is needed for a 10 mL sample and there is no risk of contaminating or degrading remaining aliquots of cocktail.
• Each microtube in the strip is heat-sealed with a foil that can be easily punctured by a pipette tip for trouble-free access to a precise amount of Protease Inhibitor Cocktail needed for each experiment.
Note:Protease Inhibitor Cocktail effectively inhibit serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. Regular and EDTA-free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis, respectively.

Product BackGround

Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids in a wide variety of cell and tissue types. Proteases are regulated and compartmentalized in living cells, but cell lysis releases and mixes them with other extracted proteins. Their activity can thus lead to the loss of a large number of valuable proteins, adversely affecting downstream applications. For this reason, it is common practice to spike cell lysis buffers or cell extracts with a cocktails of compounds known to inactivate or inhibit proteases.

Storage

This product £¨IC-7902)£© kept under 4 ¡æ for 6 months or kept under -20 ¡æ for 18months .Upon delivery aliquot,Avoid freeze / thaw cycle.

Order Size

Cata.No.

Quantity

Price($)

Price($)

Price(£¤/CNY£©

IC-7902

1ML

$57.50

€69.00

£¤575.00

IC-7902

5x1ML

$201.00

€241.20

£¤2010.00

IC-7902

10x1ML

$725.00

€870.00

£¤7250.00

Other  Information

This  product  is  offered  by  BioCytoSci only  for Scientific Research & Laboratory  USE. NOT FOR OTHER USE.

We Devoted Ourselves To The Development Of Biomedical Research Reagent.