Protein-Free Cell Cryopreservation Medium
Cat.No.:SF1800-03
Description
SF1800-03 is a Protein-Free cell cryopreservation medium with unique complete formulation for broad spectrum of mammalian cell cultures. The unique formulation allows for stable cryopreservation and high viability after freeze-thaw procedure, without potential risk of contamination serum.Optimum for Protein-Free cultured cells and peptide/protein expressing cells cryopreservation.
Advantages
-Safe: high cell viability, no risk of contamination Protein derived pathogens, batch-to-batch stability.
-Save: complete formulation, no further preparation, direct freezing at -80℃, no programmable freezer required, etc.
Size
10ml,50ml,100.0ml
Usage
Freezing
For optimum results, cells for cryopreservation should be in log phase of growth. Similar or standard freezing protocols may be substituted.
1.Examine and make sure the cell culture free of contamination, in healthy situation and proper confluency, etc.
2.Perform a cell count to determine the viability of cells.
3.Gently pellet the cells centrifugation (3-5 minutes at 1,000~2,000rpm, 4℃). Remove the supernatant using a aspirator.
4.Gently suspend the cells with SF1800-03 cryopreservation medium (1 ml for 5×105 - 5×106 cells).
5.Dispense the cell suspension in 1ml aliquots to cryopreservation vials that have been labeled with the cell line name, cell concentration, passage date and other essential information.
6.Place the vials directly in a -80℃ for storage. If necessary, transfer the frozen vials to a liquid nitrogen storage tank after the vials have been frozen for at least 24 hours.
IMPORTANT: Optimum protocol may change with the cell types.
Thawing
1. Remove the frozen cell from storage and quickly thaw in a 37℃ shaking waterbath.
2. Immediately dilute and gently mix each 1ml of cells with 10ml of complete cell culture medium.
3. Gently pellet the cells centrifugation (3-5 minutes at 1,000 - 2,000rpm, 4℃). Remove the supernatant aspirator.
4. Gently suspend the cells with appropriate volume of complete cell culture medium. And plate in a culture flask.
5. Continue the further culture procedures according to standard protocols.
Prsentation
Protein-Free formulation. Contains DMSO.
Storage and Stability
Store at 4℃ for 12months. The product will be stable for 36months under -20℃.
Quality Control
SF1800-03 cryopreservation medium is performance tested on Jurkat and NIH-3T3 cells. Additional standard evaluations are endotoxin, pH, osmolarity and tests for absences of Mycoplasma contaminants.
|
TESTS |
SPECIFICATION |
|
Endotoxin |
Not more than 7.5 EU/ml |
|
Mycoplasma |
N/A |
Note:SF1800-03 has been tested in over 100 cell lines, including but not limited to NIH-3T3，BMSC，MSC，RAW264.7，MCF-7，HepG2，AGS，HT29，HCT116，A549，C2C12， PC12，PC-9，Jurkat，THP-1，CACO2, LNCap, Hepa 1-6, etc.
Order Information
|
Cat./REF. |
Size |
Price($） |
Price(�) |
Price(￥/CNY） |
Price(￥/JYP） |
|
SF1800-03 |
10ml |
$20.00 |
� 24.00 |
￥200.00 |
￥3,900.00 |
|
SF1800-03 |
50ml |
$75.00 |
� 90.00 |
￥750.00 |
￥14,625.00 |
|
SF1800-03 |
100ml |
$122.50 |
� 147.00 |
￥1,225.00 |
￥23,887.50 |
Product Use Statement
THESE PRODUCTS ARE FOR RESEARCH USE ONLY. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro procedures.
