Western SuperSensitive Substrate(IC-8001)

DataSheet

The most sensitive ECL substrate for Western blotting analysis

Western SuperSensitive  Substrate, HRP is an extremely sensitive enhanced chemiluminescent substrate for femtogram-level by detection of immobilized proteins (Western blotting). In Western blotting or ELISA application, Western SuperSensitive  Substrate, HRP provides high signal and low background, which allows detection of target protein on PVDF or nitrocellulose. This feature benefits the researchers with excellent low background result and without signal burn effect at the same time.

Special Characteristic

High sensitivity ¨C detect femtogram of protein target on PVDF or nitrocellulose membrane

Antibody saving ¨C use very low antibody concentration on the WB and ELISA application; dilute

primary antibody 5000 to 20,000-fold and secondary antibody 100,000 to 400,000-fold (from 1mg/mL stock).

Low background ¨C provide insignificant background and avoid non-specific signals on the WB result.   

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