Puromycin Dihydrochloride(Solution)(IC-1608)

Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene.

Selection antibiotic: cell culture tested, sterile reagent

Puromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger.

It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes.

This antibiotic inhibits the growth of Gram positive bacteria and various animal and insect cells. Puromycin can also be used in some particular conditions for the selection of E. coli transformants.

Resistance to puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase [1].

Animal cells are generally sensitive to concentrations from 1 to 10 ¦Ìg/ml.

1. Lacalle R. et al., 1989. Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger. Gene. 79:375-80. 

Specifications

Product concentration: 10 mg/ml

CAS number: 58-58-2

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.

Purity: ¡Ý 98% (HPLC)

Endotoxin level: < 5 EU/mg

Physicochemical characterization: pH, appearance

Cell-culture tested: potency validated in puromycin-sensitive and puromycin‑resistant mammalian cell lines

Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in puromycin-resistant cells