Reagent
Mitochondrial Assay Kit(JC-1)IC-6569
Click£º3373 Release date£º2018-12-4 Author£ºAdministrator Source£ºOriginal
Mitochondrial Membrane Potential Assay Kit(JC-1)IC-6569
BackGround
JC1 - mitochondrial membrane potential (¦¤¦×m) kit uses tetraethylbenzimidazolylcarbocyanine iodide (JC-1),
a cationic dye that accumulates in energized mitochondria to measure the mitochondrial membrane potential.
At low concentrations (due to low ¦¤¦×m) JC-1 is predominantly a monomer that yields green fluorescence
with emission of 530¡À15 nm.At high concentrations (due to high ¦¤¦×m) the dye aggregates yielding a red
to orange colored emission (590¡À17.5 nm). Therefore, a decrease in the aggregate fluorescent count is
indicative of depolarization whereas an increase is indicative of hyperpolarization.
The accompanying FCCP (carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is an ionophore uncoupler of
oxidative phosphorylation. Treating cells with FCCP eliminates mitochondrial membrane potential and JC1
staining. JC1 is suitable for the labeling of mitochondria in live cells and it is not compatible with
fixation.
Depolarization can be found in the presence of ionophores that could induce nonselective cation channels
or become selective mobile ionic carriers. Protonophores such as FCCP and CCCP induce reversal of the ATPase,
as a compensatory mechanism that tries to maintain
¦¤¦×m, which will deplete ATP even in the presence of a normal glycolytic pathway. Hyperpolarization could
be found in the presence of ATPase inhibition, inadequate supply of ADP, increased supply of NADH, apoptosis
due to oxidative stress and potentially proton
slippage due to cytochrome c oxidase dephosphorylation. In either scenario, OXPHOS uncoupling ensues.
Membrane potential (¦¤¦×m) is highly interlinked to many mitochondrial processes. The ¦¤¦×m controls ATP
synthesis, generation of ROS,
mitochondrial calcium sequestration, import of proteins into the mitochondrion and mitochondrial membrane
dynamics. Conversely,¦¤¦×m is controlled by ATP utilization, mitochondrial proton conductance, respiratory
chain capacity and mitochondrial calcium.
On an article£ºCell Counting Kit-8 (CCK-8) IC-1519
Next article£ºPhalloidin,IC-6130,6131/6132/6133/6134/6135/6136
