DMEM (Low Glucose),IC-2031

Description

Lots of modifications of Eagle¡¯s medium have been developed since the creation of the first formulation. The most used Eagle¡¯s medium is the Dulbecco''''s Modified Eagle''''s Medium (DMEM).

It is a modification of Basal Medium Eagle (BME) that contains a concentration more important of amino acids and vitamins and also supplementary components. DMEM Low Glucose corresponds to the original formulation with 1000 mg/L of glucose; it was used to culture embryonic mouse cells.

Theoretical pH : 7.3 ¡À 0.3

Osmolality : 316 mOsm/kg ¡À  10 %

Colour : Red, clear solution

Storage conditions : +2¡ãC to +8¡ãC

Shelf life : 12 months

Sterility tests :

-Bacteria in aerobic and anaerobic conditions

-Fungi and yeasts

Endotoxins : < 1 EU/ml

Cell growth test :Medium tested for the ability to support Hela cell growth.

Composition : Displayed on website and in catalog; also available on request.

Recommended use

-Respect storage conditions of the product

-Do not use the product after its expiry date

-Store product in an area protected from light (not necessary for saline solutions).

-Manipulate the product in aseptic conditions (e.g. : under laminar air flow)

-Wear clothes adapted to the manipulation of the product to avoid contamination (e.g. : gloves, mask, hygiene cap, overall¡­)

Usage

Supplements, such as antibiotics, should be added as sterile supplements to the medium.

Storage conditions and shelf-life of supplemented product will be affected by the nature of the supplements. Sterile serum should not be refiltered before or after being added to sterile medium because growth promoting capacity may be reduced upon re-filtration.

The product is intended to be used in vitro, in laboratory only. Do not use it in therapy, human or veterinary applications.