RPMI Medium 1640(IC-2033-S)
Description
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.This product can replace SH30809.01(Hyclone).
Contents
This RPMI Medium 1640 is modified as follows:
With L-glutamine,Phenol Red
The complete formulation is available.
|
Components |
Concentration (mg/L) |
|
Sodium Bicarbonate |
2000.00 |
|
Sodium Chloride |
6000.00 |
|
Potassium Chloride |
400.00 |
|
Magnesium Sulfate |
48.84 |
|
Sodium Phosphate Dibasic, Anhydrous |
800.00 |
|
L-Arginine |
200.00 |
|
L-Asparagine |
50.00 |
|
L-Aspartic Acid |
20.00 |
|
L-Cystine-2HCl |
65.15 |
|
L-Glutamic Acid |
20.00 |
|
L-Glutamine |
300.00 |
|
Glycine |
10.00 |
|
L-Histidine F.B |
15.00 |
|
Hydroxy-L-Proline |
20.00 |
|
L-Isoleucine |
50.00 |
|
L-Leucine |
50.00 |
|
L-Lysine-HCl |
40.00 |
|
L-Methionine |
15.00 |
|
L-Phenylalanine |
15.00 |
|
L-Proline |
20.00 |
|
L-Serine |
30.00 |
|
L-Threonine |
20.00 |
|
L-Tryptophan |
5.00 |
|
L-Tyrosine-2Na-2H2O |
28.83 |
|
L-Valine |
20.00 |
|
D-Glucose (Anhydrous) |
2000.00 |
|
D-Biotin |
0.20 |
|
Calcium D-Pantothenate |
0.25 |
|
Choline Chloride |
3.00 |
|
Folic Acid |
1.00 |
|
Myo-Inositol |
35.00 |
|
Niacinamide |
1.00 |
|
Pyridoxine-HCl |
1.00 |
|
Riboflavin |
0.20 |
|
Thiamine-HCl |
1.00 |
|
Vitamin B12 |
0.01 |
|
Calcium-(NO3)2-4H2O |
0.00 |
|
Para-Aminobenzoic Acid (PABA) |
1.00 |
|
L-Glutathione (reduced) |
1.00 |
|
Phenol Red-Na |
5.30 |
Using RPMI 1640 Medium
RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B12 and PABA, which are not found in Eagles Minimal Essential Medium or Dulbeccos Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with L-glutamine minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5¨C10% CO2 environment to maintain physiological pH.
Product use
For human ex vivo tissue and cell culture processing applications.
CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using InCellGen® RPMI 1640 medium in a manufacturing process, may request from us a letter of authorization to reference our Type II Drug Master File (DMF).
Order Information
|
Cat./REF. |
Size |
Price($£© |
Price(€) |
Price(£¤/CNY£© |
Price(£¤/JYP£© |
|
IC-2032-S |
500ml |
$17.00 |
€ 20.00 |
£¤170.00 |
£¤3,700.00 |
