Lipid-Depleted Fetal Bovine Serum (LDFBS)，IC-1910
Product Description
1.Source: Fetal bovine serum (FBS) processed to remove lipids (cholesterol, phospholipids, lipoproteins) while retaining growth factors and proteins.
2.Appearance: Clear, light yellow liquid, free of precipitates .
3.Sterility: 0.1um filtered, tested negative for mycoplasma and viruses.
Key Features
1. Lipid Content: Cholesterol ≤ 5ug/mL (vs. 100–200ug/mL in standard FBS). >90% total lipid removal.
2. Retained Components: Growth factors (e.g., IGF, EGF), hormones, amino acids, vitamins.
3.Cell Compatibility: Ideal for lipid-sensitive studies (e.g., lipid metabolism, atherosclerosis models, certain cancer cell lines).
Applications
1.Lipid Metabolism Research: Study cellular responses to lipid-deficient conditions.
2.Signaling Pathways: Minimize lipid interference in receptor studies (e.g., GPCR, insulin signaling).
3.Specialized Cell Culture: For cells requiring precise lipid control or low-lipid environments.
Usage
1. Thawing: Slow thaw at 2–8°C (recommended) or rapid thaw in 37°C water bath. Avoid repeated freeze-thaw cycles.
2. Medium Preparation: Recommended concentration: 5–20% (adjust per cell requirements). Sterile-filter (0.22um) after mixing with basal medium.
3. Storage: Long-term: –20°C to –80°C; thawed aliquots stable at 2–8°C for ≤1 month.
Precautions
1.Cell Adaptation: Some cells may exhibit reduced growth; pre-testing is advised.
2.Lipid Supplementation: Add exogenous lipids (e.g., cholesterol) if needed.
3.Contamination Risk: Use aseptic techniques; aliquot to minimize contamination.
Quality Control
- Testing:
- Endotoxin ≤ 10 EU/mL.
- Hemoglobin ≤ 25 mg/dL.
- Microbiological testing (bacteria, fungi, mycoplasma): Negative.
FAQs
Q1: Can LDFBS fully replace standard FBS?
A: No. Use only for specific low-lipid studies; standard FBS is recommended for routine culture.
Q2: How to handle serum precipitates?
A: Centrifuge (2000 rpm, 5 min) for minor precipitates; contact the supplier if excessive.
Ordering Information
|
Cat./REF. |
Size |
Price($） |
Price(�) |
Price(￥/CNY） |
Price(￥/JYP） |
|
IC-1910 |
50ml |
$135.00 |
� 162.00 |
￥1,350.00 |
￥26,865.00 |
|
IC-1910 |
100ml |
$230.00 |
� 276.00 |
￥2,300.00 |
￥45,770.00 |
