RPMI Medium 1640,IC-2024-S
Product Description
RPMI Medium 1640 (Roswell Park Memorial Institute Medium) is a general-purpose cell culture medium originally developed for the cultivation of human lymphocytes in suspension. This modified formulation is specifically optimized for a broad range of mammalian cells, including various cancer cell lines (e.g., HeLa, MCF-7), leukocytes, and primary cells.
This product is provided as a sterile, ready-to-use liquid medium.
Product Features
Specifications at a glance:
- Basal Medium: RPMI 1640
- Glucose: + 2.0 g/L (high glucose)
- L-Glutamine: + 2.0 mM (stable)
- Sodium Pyruvate: – (None added)
- Phenol Red: – (Phenol Red-free)
- Sterility: 0.1 µm sterile filtered
- Volume: 500 mL
Key Advantages:
- Phenol Red-free: Ideal for experiments involving colorimetric assays (e.g., MTT, ELISA, fluorescence-based studies) where pH indicator interference must be avoided.
- High Glucose (2.0 g/L): Provides enhanced energy support for fast-proliferating cells.
- L-Glutamine Included: No need for post-thaw supplementation under normal usage.
- Sodium Pyruvate-free: Allows controlled metabolic studies or is suitable for cell lines that do not require exogenous pyruvate.
- 0.1 µm Filtration: Ensures removal of mycoplasma and other small contaminants.
Usage Instructions (For Reference Only)
A. Preparation Before Use
- Inspect the bottle for any signs of turbidity, precipitation, or color change (should be clear and colorless due to absence of Phenol Red).
- Warm the medium to 37℃ in a water bath or incubator. Do not heat above 40℃.
- Do not leave medium at 37℃ for prolonged periods (avoid >30 minutes before use).
B. Complete Medium Preparation (if supplements are required)
This basal medium contains L-Glutamine and Glucose but no serum, antibiotics, or pyruvate. Based on your cell line, aseptically add:
- Fetal Bovine Serum (FBS): Typically 5–10% (v/v)
- Penicillin-Streptomycin: 1% (optional, for contamination control)
> Note: Since this medium lacks Sodium Pyruvate, some sensitive cell lines may require supplementation with 1 mM Sodium Pyruvate (check your cell line''''s ATCC or published requirements).
C. Cell Culture Procedure
1. Aspirate old medium from the culture vessel.
2. Gently rinse cell monolayer with sterile PBS (if using adherent cells).
3. Add pre-warmed complete medium to cells.
4. Incubate at 37℃ in a humidified 5–10% CO₂ atmosphere.
5. Change medium every 2–3 days or as required by cell density.
Important Precautions
- For Research Use Only. Not intended for human therapeutic or diagnostic use.
- Sterile Technique: Use aseptic handling in a biosafety cabinet (BSC) to maintain sterility.
- Storage: Store upright at 2–8℃. Do not freeze – freezing will cause precipitation of salts and loss of functionality.
- Expiration: Do not use beyond the 12-month shelf life. Discard if the solution appears cloudy, contains particulates, or if the bottle shows signs of leakage.
- CO₂ Requirement: This medium is buffered with a sodium bicarbonate system. It requires a 5–10% CO₂ atmosphere to maintain physiological pH (typically 7.2–7.4). Do not use in open air without CO₂ enrichment.
Frequently Asked Questions
Q1: Why is there no Phenol Red? Can I still tell if the pH is correct?
A: Without Phenol Red, pH is not visually indicated. You must rely on a CO₂-regulated incubator and test pH using a calibrated pH meter or by adding a small volume of sterile Phenol Red solution if visual monitoring is required for your application.
Q2: Do I need to add L-Glutamine?
A: No. This medium already contains 2.0 mM stable L-Glutamine. However, L-Glutamine can degrade over time. For cultures longer than 2–3 weeks or very sensitive cells, consider supplementing with an additional 2 mM L-Glutamine.
Q3: My cells usually require Sodium Pyruvate. What should I do?
A: This formulation does not contain Sodium Pyruvate. You may aseptically add sterile Sodium Pyruvate solution (e.g., 100 mM stock) to a final concentration of 1 mM.
Q4: The medium looks slightly hazy after storage at 4℃. Is it still good?
A: Slight opalescence is normal for protein-free or serum-free media containing certain vitamins. However, if you see visible particles, flocs, or a white precipitate, do not use. Gently swirl the bottle at room temperature – most slight haziness will clear.
Q5: Can I use this medium for primary lymphocyte culture?
A: Yes. RPMI 1640 is classic for lymphocytes. The absence of Phenol Red and Sodium Pyruvate makes it suitable for activation studies or flow cytometry. Supplement with 10% FBS and appropriate growth factors (e.g., IL-2).
Q6: What is the shelf life once I add FBS and other supplements?
A: Complete medium (basal + FBS/antibiotics) should be used within 2–4 weeks when stored at 2–8℃, provided it is kept sterile and no turbidity develops.
Product Disclaimer
This product is for research use only. For storage of precious samples, please conduct small-scale testing prior to large-scale experiments. Product warranty is limited to the product itself and excludes any other consequential or liabilities. Please be aware of this.
For Research Use Only. Not intended for clinical diagnosis or therapeutic use.
Oder Information
|
Cat./REF. |
Size |
Price($） |
Price(�) |
Price(￥/CNY） |
Price(￥/JYP） |
|
IC-2024-S |
500ml |
$40.00 |
� 48.00 |
￥400.00 |
￥7,960.00 |
