PKH26 Staining Kit, IC-6520
Product Introduction
This product is a cell membrane labeling kit based on the lipophilic fluorescent dye PKH26. PKH26 contains long aliphatic chains that stably insert into the lipid bilayer of the cell membrane, providing stable, uniform, and non-specific fluorescent labeling of living cells.
PKH26 labeling is widely used for cell proliferation tracking, migration assays, co-culture differentiation, in vivo homing studies, and cell-cell interaction analysis. The dye binds firmly to the membrane, allowing fluorescence signal to persist for several weeks in vitro and in vivo with minimal impact on cell physiology. Its red fluorescence is compatible with green probes (e.g., GFP, FITC) for multi-color analysis.
Product Features
1. Long-term Tracking: Stable membrane integration allows fluorescence retention for weeks.
2. Uniform Staining: Low cell-to-cell variation suitable for flow cytometry.
3. Low Cytotoxicity: Minimal impact on viability, proliferation, or differentiation.
4. Spectral Compatibility: Works with blue/green probes (GFP, Hoechst) for multiplex imaging.
5. Ready-to-Use: Includes Diluent C for standardized preparation.
Specifications
Size: 0.1 mL
Storage and Stability
Storage Conditions: Store kit components at 4¡ãC or -20¡ãC, protected from light.
Shelf Life: The product is stable for 12 months from the date of manufacture when stored as directed. Avoid repeated freeze-thaw cycles.
Excitation/Emission: 551 nm / 567 nm (Red Fluorescence)
Protocol (For Reference Only)
Important: Staining must be performed under serum-free conditions.
1. Cell Preparation: Prepare a single-cell suspension, wash cells twice with serum-free medium or PBS, and resuspend pellet in Diluent C (provided) at 1x10^6 to 1x10^7 cells/mL.
2. Dye Working Solution (Prepare Fresh): Dilute PKH26 stock solution in Diluent C to achieve a final concentration of 2-4 µM during staining and mix gently.
3. Staining: Rapidly mix equal volumes of cell suspension and 2X Dye Working Solution, incubate at room temperature (25¡ãC) for 2-5 minutes, and gently invert periodically. Note: Avoid prolonged incubation.
4. Stop Reaction: Add an equal volume of Fetal Bovine Serum (FBS) or complete medium containing 10% FBS, incubate for 1 minute at RT, then centrifuge and wash cells 2-3 times with complete medium until supernatant is clear.
5. Analysis: Resuspend cells in complete medium for flow cytometry, microscopy, culture, or injection.
Precautions
1. Protect from light during handling and storage.
2. Strictly maintain serum-free conditions prior to the stopping step.
3. Use highly viable cells to minimize background from debris.
4. Optimize dye concentration for first-time use to prevent aggregation.
5. Store components at 4¡ãC or -20¡ãC protected from light. Avoid freeze-thaw cycles.
FAQ (Simplified)
Q1: Low fluorescence intensity?
A1: Check for serum contamination, dye degradation due to light exposure, or excessive cell density.
Q2: High background/particulate fluorescence?
A2: Ensure cell viability is high prior to staining and wash thoroughly after stopping the reaction.
Q3: Can labeled cells be fixed?
A3: Yes. PKH26 is compatible with standard 4% paraformaldehyde fixation.
Q4: Does it affect proliferation?
A4: Minimal impact at recommended concentrations. It is commonly used for tracking cell division via fluorescence halving.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of conditions using a small-scale pilot experiment is strongly recommended before processing valuable samples.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-6520
Product Name: PKH26 Red Fluorescent Cell Membrane Staining Kit
Size: 0.1 mL
Price: CNY ¥1500.00 / USD $150.00 / EUR €180.00 / JPY ¥27000.00
2023 Version.
