DMEM High Glucose Medium, IC-2206-S
Product Introduction
This product is a sterile-filtered Dulbecco''''s Modified Eagle Medium (DMEM) formulated with high glucose (4500 mg/L D-Glucose) to support optimal growth and metabolism of a wide variety of adherent and suspension mammalian cell lines. DMEM High Glucose is one of the most widely used basal media for cell culture, originally developed by Harry Eagle and subsequently modified by Renato Dulbecco to include higher concentrations of amino acids, vitamins, and glucose. The high glucose concentration provides an abundant energy source for rapidly dividing cells and is particularly suitable for culturing high-density cell populations, primary fibroblasts, neuronal cells, and various cancer cell lines. This complete formulation includes sodium bicarbonate buffer system, L-glutamine, sodium pyruvate, and phenol red pH indicator, providing a ready-to-use medium requiring only supplementation with fetal bovine serum and antibiotics according to specific cell line requirements.
Product Features
1. High Glucose Formulation: Contains 4500 mg/L D-Glucose, providing abundant energy substrate for high-density cultures and rapidly proliferating cells.
2. Complete Base Formulation: Includes 1500 mg/L sodium bicarbonate, 4 mM L-glutamine, 1 mM sodium pyruvate, and 15 mg/L phenol red for comprehensive nutritional support and pH monitoring.
3. Sterile and Ready-to-Use: Supplied as a sterile-filtered (0.1 µm) liquid medium requiring only serum and antibiotic supplementation before use.
4. Wide Cell Type Compatibility: Suitable for culturing fibroblasts, embryonic stem cells, neuronal cells, endothelial cells, and numerous cancer cell lines including HeLa, HEK293, and CHO derivatives.
5. Quality Tested: Each lot is tested for sterility, endotoxin levels, pH, osmolality, and cell growth promotion to ensure consistent performance.
Specifications
Size: 500 mL
Formulation: With 4500 mg/L D-Glucose, 1500 mg/L NaHCO₃, 4 mM L-Glutamine, 1 mM Sodium Pyruvate, 15 mg/L Phenol Red, without HEPES
Storage and Stability
Storage Conditions: Store at 2°C to 8°C, protected from light.
Shelf Life: The product is stable for 12 months from the date of manufacture when stored unopened as directed. Once opened, use within 4-6 weeks when stored properly and handled aseptically.
pH Range: 7.0-7.4
Osmolality: 320-360 mOsm/kg
Protocol (For Reference Only)
Important: Use aseptic technique when handling medium to prevent contamination. Warm medium to 37°C before use for optimal cell growth.
1. Preparation of Complete Medium: Supplement DMEM High Glucose with fetal bovine serum to a final concentration of 5-10% and add antibiotics as required for specific applications, then mix gently by swirling.
2. Medium Pre-warming: Place the required volume of complete medium in a 37°C water bath or incubator for 15-30 minutes before use to minimize thermal shock to cells.
3. Cell Feeding: Aspirate spent medium from culture vessel, replace with fresh pre-warmed complete DMEM High Glucose, and return cells to the incubator maintained at 37°C with 5-10% CO₂.
4. Subculturing: When cells reach 80-90% confluency, detach adherent cells using trypsin-EDTA, neutralize with complete medium, centrifuge if required, and resuspend in fresh complete DMEM High Glucose for seeding into new vessels.
5. Monitoring: Observe phenol red color indicator daily to assess pH changes; medium should appear pink-red at physiological pH and turn yellow-orange as acidity increases from cellular metabolism.
Precautions
1. Store medium at 2-8°C and protect from light to prevent degradation of light-sensitive components including L-glutamine and phenol red.
2. Avoid repeated warming and cooling cycles; aliquot medium into smaller volumes for daily use to maintain sterility and component stability.
3. Supplemented complete medium should be used within 2-4 weeks when stored at 4°C as glutamine degrades and antibiotic activity diminishes over time.
4. Monitor osmolality changes if medium is stored beyond recommended period or exposed to evaporation.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the difference between DMEM High Glucose and DMEM Low Glucose?
A1: DMEM High Glucose contains 4500 mg/L D-Glucose, whereas DMEM Low Glucose contains 1000 mg/L. High glucose formulations are preferred for rapidly dividing cells, high-density cultures, and cell lines with high glycolytic activity, while low glucose formulations are more physiological for primary cells and metabolically sensitive cultures.
Q2: Does this medium contain L-glutamine and should I add additional glutamine?
A2: This medium is supplied with 4 mM L-glutamine. For long-term culture or glutamine-sensitive cell lines, additional supplementation may be beneficial as glutamine degrades over time at 4°C and 37°C. Consider using stable glutamine substitutes for extended culture periods.
Q3: Why does my medium color change from pink to yellow rapidly after culturing?
A3: Phenol red is a pH indicator that turns yellow in acidic conditions. Rapid yellowing indicates active cellular metabolism producing lactic acid, which is normal for high-density or rapidly proliferating cultures. Change medium more frequently if pH drops below 7.0.
Q4: Is this medium suitable for primary cell culture?
A4: Yes, DMEM High Glucose supports many primary cell types including fibroblasts, endothelial cells, and some epithelial cells. However, certain primary cells may prefer low glucose or specialized medium formulations. Consult literature for cell type-specific recommendations.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of culture conditions including serum concentration and supplementation is strongly recommended for specific cell lines.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data resulting from product use or storage.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-2206-S
Product Name: DMEM High Glucose Medium
Size: 500 mL
Price: CNY ¥400.00 / USD $40.00 / EUR �48.00 / JPY ¥7200.00
