Mouse Cardiac Fibroblast Immortalized Cell Culture Medium, CM-IMCF
Product Introduction
This product is a specialized, ready-to-use complete medium optimized for the culture and expansion of immortalized mouse cardiac fibroblasts. Cardiac fibroblasts are the most abundant non-myocyte cell type in the heart and play essential roles in maintaining the cardiac extracellular matrix, responding to myocardial injury, and mediating cardiac fibrosis. Immortalized cardiac fibroblast cell lines provide a reproducible and genetically tractable in vitro model for studying fibroblast biology, fibrotic signaling, myocardial remodeling, and cell-cell interactions in the heart without the donor variability and limited replicative capacity associated with primary cardiac fibroblasts. This medium is specifically formulated with an optimized combination of basal medium, fetal bovine serum, and growth supplements to support robust proliferation, maintain characteristic fibroblast morphology, and preserve the functional properties of immortalized mouse cardiac fibroblasts, including expression of fibroblast-specific markers, collagen synthesis, and contractile activity.
Product Features
1. Optimized for Immortalized Mouse Cardiac Fibroblasts: Specifically formulated to support robust proliferation and preserve the phenotypic characteristics of immortalized mouse cardiac fibroblast cell lines.
2. Ready-to-Use Complete Medium: Supplied as a sterile, complete formulation requiring no additional serum or growth factor supplementation, reducing preparation time and variability.
3. Preservation of Fibroblast Characteristics: Maintains spindle-shaped fibroblast morphology, expression of fibroblast markers (vimentin, collagen I, α-SMA), and functional properties.
4. Consistent Performance: Each lot is tested for sterility, pH, osmolality, and cell growth promotion to ensure batch-to-batch reproducibility.
5. Convenient: Eliminates the need for medium optimization and supplementation, providing a standardized culture system for cardiac fibrosis research.
Specifications
Size: Available in 100 mL and 500 mL
Product Form: Liquid, sterile
Cell Type: Immortalized mouse cardiac fibroblasts
Storage and Stability
Storage Conditions: Store at 4°C, protected from light. For long-term storage, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles.
Shelf Life: The product is stable for 12 months from the date of manufacture when stored as directed. After opening, use within 4-6 weeks when stored at 4°C and handled aseptically.
Protocol (For Reference Only)
Important: Use aseptic technique when handling medium to prevent contamination. Warm medium to 37°C before use for optimal cell growth.
1. Medium Preparation: Warm Mouse Cardiac Fibroblast Immortalized Cell Culture Medium to 37°C in a water bath or incubator for 15-30 minutes before use. Do not add additional serum or supplements as this medium is a complete formulation.
2. Cell Thawing: Thaw frozen cells rapidly in a 37°C water bath. Transfer the cell suspension to a centrifuge tube containing 5-10 mL of pre-warmed medium. Centrifuge at 200 x g for 5 minutes, discard supernatant, resuspend in fresh pre-warmed medium, and seed into appropriate culture vessels.
3. Routine Culture: Incubate cells at 37°C in a humidified 5% CO₂ incubator. Replace medium every 2-3 days or when the pH indicator (phenol red) shifts from pink-red to yellow-orange.
4. Subculturing: When cells reach 80-90% confluency, aspirate medium, wash with PBS, and detach cells using 0.25% trypsin-EDTA at 37°C for 2-3 minutes. Neutralize trypsin with fresh complete medium, centrifuge at 200 x g for 5 minutes, resuspend in fresh medium, and seed at an appropriate split ratio (typically 1:3 to 1:5).
5. Cryopreservation: For cell banking, harvest cells at 80-90% confluency, centrifuge at 200 x g for 5 minutes, resuspend in freezing medium (complete medium supplemented with 5-10% DMSO), aliquot into cryovials, and freeze at -80°C overnight before transferring to liquid nitrogen for long-term storage.
Precautions
1. This medium is a complete formulation; do not add additional serum or growth factor supplements as this may alter the optimized formulation and affect cell growth characteristics.
2. Avoid repeated warming and cooling cycles; aliquot medium into smaller volumes for daily use.
3. Use aseptic technique when handling to maintain sterility.
4. For research use only. Not for use in diagnostic or therapeutic procedures.
5. This product is intended for use with immortalized mouse cardiac fibroblast cell lines; it has not been validated for primary cardiac fibroblasts or fibroblasts from other species.
FAQ (Simplified)
Q1: Can I use this medium for primary mouse cardiac fibroblasts?
A1: This medium is specifically formulated for immortalized mouse cardiac fibroblasts. Primary cardiac fibroblasts may have different growth factor and nutritional requirements. It is recommended to use a medium specifically validated for primary cardiac fibroblasts or to verify compatibility by pilot experiments.
Q2: What is the recommended split ratio for subculturing?
A2: Immortalized mouse cardiac fibroblasts are typically subcultured at a split ratio of 1:3 to 1:5 when cells reach 80-90% confluency. Optimize the split ratio based on the growth characteristics of your specific cell line.
Q3: How should I cryopreserve these cells?
A3: Harvest cells at 80-90% confluency, resuspend in freezing medium (this complete medium supplemented with 5-10% DMSO), aliquot into cryovials at a density of 1-2×10⁶ cells/mL, freeze at -80°C overnight in a controlled-rate freezing container, and transfer to liquid nitrogen for long-term storage.
Q4: What fibroblast markers should be expressed by cells cultured in this medium?
A4: Cells cultured in this medium should maintain expression of fibroblast markers including vimentin, collagen type I, and under certain conditions α-smooth muscle actin (α-SMA). The expression of these markers can be verified by immunofluorescence, Western blot, or flow cytometry.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of culture conditions may be required for specific immortalized cell lines.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: CM-IMCF
Product Name: Mouse Cardiac Fibroblast Immortalized Cell Culture Medium
Size: 100 mL / 500 mL
Price:
100 mL: CNY ¥980.00 / USD $98.00 / EUR �117.60 / JPY ¥17640.00
500 mL: CNY ¥3550.00 / USD $355.00 / EUR �426.00 / JPY ¥63900.00
2023 Version
