0.45 µm PVDF Blotting Membrane, IC-8245
Product Introduction
This product is a polyvinylidene fluoride (PVDF) blotting membrane with a pore size of 0.45 µm, designed for protein transfer and immunodetection in Western blotting applications. PVDF membranes offer high protein binding capacity, mechanical strength, and chemical resistance, making them the preferred choice for Western blotting, protein sequencing, and other protein analysis techniques. The 0.45 µm pore size is suitable for proteins with molecular weights above 20 kDa, providing an optimal balance of binding capacity and low background. The membrane is supplied in convenient roll format, allowing users to cut membranes to the desired size. PVDF membranes must be pre-wetted with methanol before use to ensure proper hydration and protein binding.
Product Features
1. High Protein Binding Capacity: Hydrophobic PVDF surface provides strong protein binding for sensitive detection.
2. Mechanical Strength: Durable membrane suitable for stripping and reprobing.
3. Chemical Resistance: Compatible with organic solvents, acids, and bases used in protein chemistry.
4. Convenient Roll Format: Allows flexible sizing for different gel dimensions.
5. Broad Compatibility: Compatible with chromogenic, chemiluminescent, and fluorescent detection methods.
Specifications
Size: 30 cm ¡Á 2 m / roll; 30 cm ¡Á 4 m / roll
Pore Size: 0.45 µm
Material: Polyvinylidene fluoride (PVDF)
Application: Western blotting, protein transfer, immunodetection
Storage and Stability
Storage Conditions: Store at room temperature in a dry environment, protected from dust and direct sunlight.
Shelf Life: The product is stable for 60 months from the date of manufacture when stored as directed.
Protocol (For Reference Only)
Important: PVDF membrane must be pre-wetted with 100% methanol before use. Do not allow the membrane to dry out during the transfer and detection procedure.
1. Membrane Activation: Immerse the PVDF membrane in 100% methanol for 15-30 seconds until the membrane becomes uniformly translucent. Transfer the membrane to deionized water and rinse briefly, then equilibrate in transfer buffer for at least 5 minutes.
2. Transfer Assembly: Assemble the transfer sandwich according to standard Western blot protocols, placing the activated PVDF membrane against the gel.
3. Protein Transfer: Perform electrophoretic transfer according to standard protocols using appropriate transfer buffer (e.g., Towbin buffer with or without methanol).
4. Post-Transfer Processing: After transfer, proceed directly to blocking and immunodetection. If desired, Ponceau S staining may be performed to assess transfer efficiency.
5. Stripping and Reprobing: PVDF membranes can be stripped of antibodies using appropriate stripping buffers and reprobed multiple times.
Precautions
1. Always pre-wet PVDF membrane with methanol before use; failure to do so will result in poor protein binding.
2. Do not allow the membrane to dry out during transfer and detection steps; if drying occurs, re-wet with methanol and re-equilibrate.
3. Wear gloves and use clean forceps to handle the membrane to avoid contamination.
4. Cut membranes to size before activation for clean edges and optimal results.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: Why is methanol activation necessary for PVDF membranes?
A1: PVDF is naturally hydrophobic. Methanol activation hydrates the membrane, allowing it to interact with aqueous transfer buffer and bind proteins effectively.
Q2: What protein sizes are suitable for 0.45 µm PVDF membrane?
A2: The 0.45 µm pore size is recommended for proteins above 20 kDa. For proteins below 20 kDa, a 0.2 µm membrane is recommended to prevent protein loss through the membrane.
Q3: Can PVDF membranes be reused for multiple antibody detections?
A3: Yes. PVDF membranes can be stripped and reprobed multiple times due to their mechanical strength and chemical resistance.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of transfer and detection conditions is recommended.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-8245
Product Name: 0.45 µm PVDF Blotting Membrane
Size: 30 cm ¡Á 2 m / roll; 30 cm ¡Á 4 m / roll
Suggested Retail Price:
30 cm ¡Á 2 m / roll: CNY ¥2200.00 / USD $220.00 / EUR €264.00 / JPY ¥39600.00
30 cm ¡Á 4 m / roll: CNY ¥3340.00 / USD $334.00 / EUR €400.80 / JPY ¥60120.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
