Recombinant Human Insulin Solution (10 mg/mL), IC-1356
Product Introduction
This product is a sterile, ready-to-use recombinant human insulin solution at a concentration of 10 mg/mL, designed for use as a cell culture supplement. Insulin is a peptide hormone that plays a critical role in regulating glucose uptake, protein synthesis, and cell proliferation. In cell culture, insulin is widely used as a growth factor supplement in serum-free and low-serum media for a variety of mammalian cell types including CHO cells, HEK293 cells, hybridomas, and stem cells. Recombinant human insulin is produced using recombinant DNA technology, eliminating the risk of animal-derived contaminants and ensuring consistent lot-to-lot quality. This solution is sterile-filtered and ready for direct addition to culture medium.
Product Features
1. Recombinant Human Insulin: Produced by recombinant technology, free of animal-derived components.
2. Ready-to-Use Solution: Supplied as a sterile 10 mg/mL solution for direct use.
3. Cell Culture Grade: Suitable for serum-free and low-serum cell culture applications.
4. Consistent Quality: Recombinant production ensures batch-to-batch consistency.
Specifications
Size: 1 mL
Concentration: 10 mg/mL
Form: Sterile solution
Application: Cell culture supplement
Source: Recombinant human insulin
Storage and Stability
Storage Conditions: Store at 4¡ãC for short-term use (up to 3 months). For long-term storage, aliquot and store at -20¡ãC. Avoid repeated freeze-thaw cycles.
Shelf Life: The product is stable for 12 months from the date of manufacture when stored as directed.
Protocol (For Reference Only)
Important: Use aseptic technique when handling. Avoid repeated freeze-thaw cycles.
1. Working Concentration: The typical working concentration of insulin in cell culture medium ranges from 5-10 µg/mL. For specific cell types, concentrations may vary; consult relevant literature or optimize empirically.
2. Medium Supplementation: Add the appropriate volume of insulin solution to culture medium under aseptic conditions. For a final concentration of 10 µg/mL, add 1 µL of solution per 1 mL of medium.
3. Storage of Supplemented Medium: Culture medium containing insulin should be stored at 4¡ãC and used within 2-4 weeks.
4. Thawing: Thaw at 4¡ãC or room temperature. Mix gently by pipetting or inversion. Do not vortex.
Precautions
1. For research use only. Not for use in diagnostic or therapeutic procedures.
2. Use aseptic technique to maintain sterility after opening.
3. Avoid repeated freeze-thaw cycles; aliquot into single-use volumes upon first thaw.
4. If precipitation occurs, mix gently. Do not heat.
5. Wear appropriate protective clothing and gloves when handling this product.
FAQ (Simplified)
Q1: What is the recommended working concentration for cell culture?
A1: The typical working concentration is 5-10 µg/mL. Optimize for specific cell types.
Q2: How should I store this product?
A2: Store at 4¡ãC for short-term use (up to 3 months). For long-term storage, aliquot and store at -20¡ãC. Avoid repeated freeze-thaw cycles.
Q3: Can this insulin be used in serum-free medium?
A3: Yes. This product is suitable for serum-free and low-serum cell culture applications.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
3. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-1356
Product Name: Recombinant Human Insulin Solution (10 mg/mL)
Size: 1 mL
Suggested Retail Price: CNY ¥560.00 / USD $56.00 / EUR €67.20 / JPY ¥10080.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
