Transwell Insert, 24-Well, 8.0 µm, PET Membrane, IC-21024-80
Product Introduction
This product is a sterile, ready-to-use Transwell insert system consisting of 24-well cell culture inserts with transparent polyethylene terephthalate (PET) track-etched membranes of 8.0 µm pore size. Transwell inserts provide a permeable support for cell growth, enabling researchers to create a two-compartment culture system with independent access to the apical and basolateral sides of the cell monolayer. The PET membrane allows cells to grow on a porous surface while permitting free diffusion of nutrients, signaling molecules, and pharmacological agents across the membrane barrier. The 8.0 µm pore size is optimized for cell migration and invasion assays involving larger cell types, including endothelial cells, fibroblasts, immune cells, and metastatic cancer cells. The transparent PET membrane enables direct microscopic visualization of cells during and after culture without the need to remove the membrane. Each insert is individually packaged and supplied sterile, ensuring convenience and reproducibility for high-throughput applications.
Product Features
1. Track-Etched PET Membrane: Uniform 8.0 µm pores provide consistent and reproducible results for cell migration and invasion assays.
2. Transparent Membrane: Allows direct microscopic observation of cell morphology, monolayer formation, and migration progress without membrane removal.
3. Optimized for Larger Cell Types: 8.0 µm pore size is suitable for endothelial cells, fibroblasts, immune cells, and large cancer cells.
4. Tissue Culture Treated: PET membrane surface is optimally treated for cell attachment and growth.
5. Sterile and Ready-to-Use: Each plate is individually packaged and gamma-sterilized, eliminating the need for pre-treatment or sterilization.
Specifications
Size: 48 inserts per box
Membrane Material: Polyethylene terephthalate (PET)
Membrane Diameter: 6.5 mm
Pore Size: 8.0 µm
Plate Format: 24-well plate
Growth Area: 0.33 cm² per insert
Membrane Type: Transparent
Storage and Stability
Storage Conditions: Store at room temperature (15-25¡ãC) in a dry environment, protected from light and moisture.
Shelf Life: The product is stable for 36 months from the date of manufacture when stored as directed in unopened packaging.
Protocol (For Reference Only)
Important: Use aseptic technique when handling inserts and plates to maintain sterility. Pre-warm culture medium to 37¡ãC before use. Pre-wet the membrane with culture medium before seeding cells to ensure uniform hydration.
1. Plate and Insert Preparation: Open the sterile packaging in a biosafety cabinet, remove the 24-well plate containing inserts, and label appropriately. For cell attachment optimization, pre-coat the PET membrane with extracellular matrix proteins such as fibronectin, collagen, or laminin if required for specific cell types.
2. Cell Seeding for Migration Assays: Harvest and count cells according to standard protocols. Resuspend cells in serum-free or low-serum medium and seed into the upper chamber (insert) at a density of 1¡Á10⁴ to 1¡Á10⁵ cells per insert in 100-200 µL volume. Add 500-750 µL of complete medium containing chemoattractant (e.g., 10% FBS, growth factors, or specific chemokines) to the lower chamber (well).
3. Incubation: Incubate the plate at 37¡ãC in a humidified CO₂ incubator for the desired migration period, typically 4-24 hours for migration assays or 24-72 hours for invasion assays with extracellular matrix coating.
4. Quantification of Migrated Cells: After incubation, carefully remove the insert from the well. Remove non-migrated cells from the upper surface of the membrane using a cotton swab moistened with PBS. Fix migrated cells on the lower surface with 4% paraformaldehyde or 100% methanol for 10-15 minutes. Stain cells with 0.1% crystal violet solution for 10-20 minutes, rinse with PBS, and air dry. Quantify migrated cells by counting under a microscope in multiple random fields per insert, or elute the dye with 10% acetic acid and measure absorbance at 560-590 nm.
5. Invasion Assays: For invasion assays, coat the upper membrane surface with a thin layer of Matrigel or other extracellular matrix solution and allow to polymerize at 37¡ãC for 1-2 hours before cell seeding.
Precautions
1. Handle inserts gently using fine-tipped forceps to avoid damaging the PET membrane.
2. Avoid touching the membrane surface with pipette tips during cell seeding, medium addition, or sampling to prevent membrane puncture.
3. Pre-wet the membrane with culture medium for 10-15 minutes before cell seeding to ensure uniform hydration and cell distribution.
4. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What cell types are suitable for 8.0 µm pore size?
A1: The 8.0 µm pore size is recommended for larger cell types including endothelial cells, fibroblasts, macrophages, neutrophils, and large cancer cells. For smaller epithelial cells, 3.0 µm or 5.0 µm pore sizes may be more appropriate.
Q2: What is the recommended cell seeding density?
A2: Typical seeding density is 1¡Á10⁴ to 1¡Á10⁵ cells per insert. Optimize for your specific cell type and assay duration.
Q3: Can I reuse the inserts?
A3: No. Inserts are designed for single use only.
Q4: What is the difference between 8.0 µm and 3.0 µm Transwell inserts?
A4: The 8.0 µm pore size allows passage of larger cells and is suitable for endothelial, immune, and large cancer cell migration. The 3.0 µm pore size is suitable for smaller epithelial and cancer cell migration.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of cell seeding density and incubation conditions is recommended for specific cell types.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-21024-80
Product Name: Transwell Insert, 24-Well, 8.0 µm, PET Membrane, 48/Box
Size: 48 inserts per box
Suggested Retail Price: CNY ¥2150.00 / USD $215.00 / EUR €258.00 / JPY ¥38700.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
