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Primary Antibody Dilution Buffer(IC-8010)
Click£º4025     Release date£º2008-4-18    Author£ºAdministrator    Source£ºOriginal
Primary Antibody Dilution Buffer£šIC-8010£©

BACKGOUND 

This product is the latest generation of highly effective anti-diluent. It can increase the activity and stability of antibodies, reduce the non-specific binding of antibodies, reduce the non-specific background coloring, and improve the specificity and sensitivity of the test by adding a variety of antibody protective agents. The overall effect is significantly superior to the traditional primary antibody solution based on BSA (bovine serum albumin), skimmed milk powder and so on. It can be used for the dilution and preparation of the first anti in Western Blot.

Dilution Buffer Content

-1%BSA (blocking & stabilizer)

-0.1% cold fish skin gelatin (blocking)
-0.5% Triton X-100 (penetration enhancer)
-0.05% sodium azide (preservative)
-0.01M PBS, pH 7.2-7.4

FEATURES
-Stable and efficient - the diluted and prepared first antibody can be stored and used at 4 C for no less than 6 months, and can be repeatedly used in Western Blot, which is conducive to saving first antibody.
-Low background- no serum and albumin, so as to ensure high signal to noise ratio
-Convenience and Rapidity - Diluting the first antibody directly without any additional reagents.

STORAGE

Mix well and store at 4 oC.

Note: Do not use it to dilute HRP conjugated antibody as the sodium azide is inhibitor of HRP. 

Do not use it to dilute Alkaline Phosphatase (AP) conjugated antibody as the phosphate in PBS is an inhibitor of AP.

ORDER INFORMATION


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