Cell Stimulation Cocktail, 500X, F300112
(with Protein Transport Inhibitors)
Product Introduction
This product is a 500X concentrated cell stimulation cocktail containing phorbol 12-myristate 13-acetate (PMA), ionomycin, Brefeldin A, and monensin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types and stimulate cytokine production. Brefeldin A and monensin cause accumulation of secretory proteins in the endoplasmic reticulum and Golgi apparatus by inhibiting intracellular protein transport. Therefore, this Cell Stimulation Cocktail (with Protein Transport Inhibitors) can be used for the induction of cytokines and other secretory proteins in in vitro cultured and ex vivo cells, followed by intracellular detection.
Product Features
1. Combined Stimulation and Transport Inhibition: Contains PMA, ionomycin, Brefeldin A, and monensin in a single ready-to-use cocktail.
2. Effective Cell Activation: PMA and ionomycin induce activation and cytokine production in many cell types.
3. Protein Transport Inhibition: Brefeldin A and monensin block secretion, causing accumulation of cytokines and secretory proteins for intracellular detection.
4. Convenient 500X Concentrate: Simple dilution into culture medium for immediate use.
5. Versatile Application: Suitable for in vitro cultured cells and ex vivo cell samples for intracellular cytokine staining.
Specifications
Size: 100 µL
Concentration: 500X
Components: PMA, ionomycin, Brefeldin A, monensin
Application: Induction of cytokines and secretory proteins for intracellular detection
Storage and Stability
Storage Conditions: Store at -20¡ãC, protected from light. Avoid repeated freeze-thaw cycles. Aliquot into single-use volumes upon first use.
Shelf Life: The product is stable for 12 months from the date of manufacture when stored as directed.
Protocol (For Reference Only)
Important: Prepare working solution fresh before use. Protect from light during handling and incubation.
1. Working Solution Preparation: Dilute the 500X Cell Stimulation Cocktail 1:500 into cell culture medium to prepare 1X working solution. For example, add 2 µL of 500X cocktail to 1 mL of culture medium.
2. Cell Stimulation: Adjust cells to an appropriate density in culture medium. Add the 1X working solution to the cell suspension. Incubate at 37¡ãC, 5% CO₂ for 4-6 hours. The optimal incubation time may vary depending on cell type and target cytokine.
3. Harvesting: After incubation, harvest cells for subsequent intracellular cytokine staining. Wash cells with PBS or staining buffer.
4. Intracellular Cytokine Staining: Perform cell surface staining if required, followed by fixation and permeabilization using an appropriate Fix & Perm kit. Stain with fluorophore-conjugated anti-cytokine antibodies according to standard protocols.
5. Analysis: Analyze stained cells by flow cytometry or other appropriate detection methods.
Precautions
1. Protect from light during handling and storage; Brefeldin A and monensin are light-sensitive.
2. Prepare working solution fresh before use; do not store diluted working solution.
3. Avoid repeated freeze-thaw cycles; aliquot into single-use volumes upon first use.
4. The optimal stimulation time and concentration may vary depending on cell type and target cytokine; optimization is recommended.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the purpose of this cocktail?
A1: PMA and ionomycin activate cells and induce cytokine production, while Brefeldin A and monensin block protein secretion, causing cytokines to accumulate intracellularly for detection by flow cytometry.
Q2: What is the recommended stimulation time?
A2: Typically 4-6 hours at 37¡ãC. The optimal time may vary depending on cell type and target cytokine.
Q3: Can this cocktail be used for intracellular cytokine staining?
A3: Yes. This cocktail is specifically designed for inducing cytokine production and retaining cytokines intracellularly for subsequent intracellular staining and flow cytometric analysis.
Q4: How should I store this product?
A4: Store at -20¡ãC protected from light. Aliquot into single-use volumes and avoid repeated freeze-thaw cycles.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of stimulation conditions is recommended for specific cell types and target cytokines.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: F300112
Product Name: Cell Stimulation Cocktail (with Protein Transport Inhibitors), 500X
Size: 100 µL
Suggested Retail Price: CNY ¥1300.00 / USD $130.00 / EUR €156.00 / JPY ¥23400.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
