Cell-Del® Clodronate Liposomes, IC-0283519

Product Introduction

Macrophages are highly involved in maintaining physiological homeostasis and orchestrating pathological tissue responses. Macrophages exhibit extensive morphological and functional heterogeneity across physiological and pathological contexts including muscle motor control, cellular and vascular aging, obesity-related epigenetic imprinting, hematopoietic clonal quality control, infection and inflammation, tumor initiation and progression, metabolic and chronic diseases, and epigenetic reprogramming. The morphology and function of macrophages at early, intermediate, and late stages reflect a complex, dynamic, and continuous biological response process regulated in time and space by a series of immunological network microenvironments.

Clodronate liposomes are a widely used reagent for macrophage depletion. By encapsulating clodronate within liposomes, the phagocytic properties of macrophages are exploited to enable uptake of clodronate liposomes. After phagocytosis, clodronate is released within the macrophage lysosome through the action of lysosomal phosphatases. Accumulation of clodronate beyond a threshold specifically induces macrophage apoptosis, thereby depleting macrophages in tissues.

Control liposomes have the same properties as clodronate liposomes except that they do not encapsulate the active pharmaceutical ingredient clodronate. After being phagocytosed by macrophages, control liposomes do not induce macrophage apoptosis or depletion and serve primarily as a negative experimental control.

The Cell-Del® Clodronate Liposome Macrophage Depletion Set contains one vial of Clodronate Liposomes and one vial of Control Liposomes of equal volume. Administration routes are flexible and include intravenous, intraperitoneal, subcutaneous, intranasal, and intratracheal routes. In vivo injection doses should be determined based on animal body weight, injection frequency, administration route, and experimental objectives.

Product Features

1. Efficient Macrophage Depletion: Clodronate encapsulated in liposomes is phagocytosed by macrophages and induces specific apoptosis upon lysosomal release.

2. Flexible Administration Routes: Suitable for intravenous, intraperitoneal, subcutaneous, intranasal, and intratracheal administration.

3. Matched Control Included: The set includes equal volumes of clodronate liposomes and control liposomes for negative control experiments.

4. Uniform Particle Size: 1.5-2 µm liposomes optimized for macrophage phagocytosis.

5. Ready-to-Use Suspension: Supplied as a milky white suspension in PBS, pH 7.4.

Specifications

Size: 10 mL

Appearance: Milky white liquid

Encapsulated Drug: Clodronate disodium

Concentration: 5 mg/mL

Particle Size: 1.5-2 µm

Buffer: PBS, pH 7.4

Set Contents: Clodronate Liposomes (one vial) and Control Liposomes (one vial), equal volumes

Storage and Stability

Shipping Conditions: Shipped on ice packs.

Storage Conditions: Store at 4-8¡ãC. Do not freeze.

Shelf Life: The product is stable for 12 months from the date of manufacture when stored as directed.

Protocol (For Reference Only)

Important: This product is a suspension. Invert several times before use to ensure uniform mixing. Do not aliquot. Do not freeze. Avoid contact with organic reagents and surface detergents.

1. Preparation: Remove the vial from 4-8¡ãC storage and invert several times to resuspend the liposomes uniformly.

2. Administration Route Selection: Select the appropriate administration route based on experimental objectives (intravenous, intraperitoneal, subcutaneous, intranasal, or intratracheal).

3. Dose Determination: Determine the injection dose based on animal body weight, injection frequency, administration route, and experimental objectives. Consult published literature for reference doses.

4. Injection: Draw the suspension using a sterile syringe under aseptic conditions. Inject according to the selected route.

5. In Vitro Experiments: For in vitro experiments, the liposome suspension may be diluted with sterile PBS.

Precautions

1. Store at 4-8¡ãC. Do not freeze.

2. Do not expose to organic reagents or surface detergents, which may disrupt the liposome membrane structure.

3. The product is a suspension and may settle upon standing. Invert several times before use to ensure uniform mixing.

4. Do not aliquot. For in vitro experiments, dilute with sterile PBS as needed.

5. Draw with a sterile syringe and maintain aseptic technique to avoid contamination.

6. For research use only. Not for use in diagnostic or therapeutic procedures.

FAQ (Simplified)

Q1: How do clodronate liposomes deplete macrophages?

A1: Macrophages phagocytose clodronate liposomes, and clodronate is released within lysosomes. Accumulation of clodronate beyond a threshold specifically induces macrophage apoptosis.

Q2: What is the purpose of the control liposomes?

A2: Control liposomes have the same properties as clodronate liposomes but do not contain clodronate. They serve as a negative control and do not induce macrophage depletion.

Q3: How should I store this product?

A3: Store at 4-8¡ãC. Do not freeze. Invert several times before use to resuspend.

Q4: Can I aliquot this product?

A4: No. Aliquoting is not recommended. For in vitro experiments, dilute with sterile PBS as needed.

Disclaimer

1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.

2. Due to the variable nature of biological research, optimization of injection dose and route is recommended for specific animal models and experimental objectives.

3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.

4. Wear appropriate protective clothing and gloves when handling this product.

Product Statement

This product is manufactured by Biotarget (Beijing) Co., Ltd. under an authorized partnership. Cell-Del® is a registered trademark of Biotarget (Beijing) Co., Ltd.

Ordering Information

Catalog Number: IC-0283519

Product Name: Cell-Del® Clodronate Liposomes

Size: 10 mL

Suggested Retail Price: CNY ¥6150.00 / USD $615.00 / EUR €738.00 / JPY ¥110700.00

Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.