Endothelial Cell Culture Medium, CM-ECM
Product Introduction
This product is a specialized complete medium formulated to maintain the optimal growth state of endothelial cells. Endothelial cells line the inner surface of blood vessels and play essential roles in vascular homeostasis, angiogenesis, barrier function, and inflammation. This medium contains all components required for the growth of endothelial cells, including basal medium, fetal bovine serum, and endothelial cell growth supplements, and requires no additional supplementation before use. It is suitable for the culture and expansion of endothelial cells in vascular biology, angiogenesis research, and cardiovascular studies.
Product Features
1. Ready-to-Use Complete Medium: Contains all necessary components; no additional supplementation required.
2. Optimized for Endothelial Cells: Specifically formulated to support the growth and maintenance of endothelial cells.
3. Quality Controlled: Each batch tested for sterility, pH, endotoxin, and cell growth performance.
4. Serum-Containing Formulation: Includes fetal bovine serum for comprehensive nutritional support.
5. Broad Application: Suitable for vascular biology, angiogenesis, and cardiovascular research.
Specifications
Size: 100 mL / 500 mL
Cell Type: Endothelial cells
Form: Liquid complete medium
Storage and Stability
Storage Conditions: Store at 2-8°C, protected from light for short-term storage. For long-term storage, store at -20°C, protected from light.
Shelf Life: 2 months at 2-8°C; 6 months at -20°C.
Quality Control
Clarity: Clear
pH: 7.3 ± 0.2
Endotoxin: ≤10 EU/mL
Sterility: Negative for bacteria, fungi, and mycoplasma
Cell Growth Test: Cell morphology normal; cell growth test qualified
Protocol (For Reference Only)
Important: Use aseptic technique throughout. Warm complete medium to 37°C before use.
1. Complete Medium Preparation: If components are provided separately, thaw the supplement and FBS at 4°C or room temperature, then add to the basal medium under aseptic conditions. Mix gently by swirling. Store the prepared complete medium at 2-8°C protected from light and use within 2 months.
2. Medium Pre-warming: Warm the required volume of complete medium to 37°C for 15-30 minutes before use.
3. Cell Culture: Seed cells in appropriate culture vessels pre-coated with extracellular matrix if required (e.g., fibronectin, gelatin, or collagen). Incubate at 37°C, 5% CO₂.
4. Medium Change: Replace medium every 2-3 days or when pH indicator changes color.
5. Subculturing: When cells reach 80-90% confluency, detach using trypsin-EDTA or other appropriate dissociation reagent and passage according to standard protocols for endothelial cells.
Precautions
1. Upon receipt, inspect the packaging for integrity. If breakage, leakage, or turbidity is observed, contact the supplier promptly. After freeze-thaw, small amounts of flocculent precipitate may appear in the medium; this does not affect normal product use.
2. Read the product manual carefully to understand usage, storage, and shelf life. Contamination due to improper operation or storage is the responsibility of the customer.
3. This product is for research use only. Some components in the medium have low toxicity to humans. If contact occurs, rinse immediately with copious amounts of water.
FAQ (Simplified)
Q1: Does this medium require any additional supplementation?
A1: No. This complete medium contains all components required for endothelial cell culture and requires no additional supplementation.
Q2: Should culture vessels be coated before seeding endothelial cells?
A2: Endothelial cells typically attach better on surfaces pre-coated with extracellular matrix proteins such as fibronectin, gelatin, or collagen. Coating is recommended for optimal attachment and growth.
Q3: How should I store this medium?
A3: Store at 2-8°C protected from light for 2 months, or at -20°C protected from light for 6 months.
Q4: What should I do if flocculent precipitate appears after thawing?
A4: Small amounts of flocculent precipitate may appear after freeze-thaw and do not affect product performance. Mix gently before use.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data resulting from improper use or storage.
3. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: CM-ECM
Product Name: Endothelial Cell Culture Medium
Size: 100 mL / 500 mL
Suggested Retail Price:
100 mL: CNY ¥1200.00 / USD $120.00 / EUR �144.00 / JPY ¥21600.00
500 mL: CNY ¥2850.00 / USD $285.00 / EUR �342.00 / JPY ¥51300.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
