(-)-Epigallocatechin Gallate, IC-01220164
Product Introduction
(-)-Epigallocatechin gallate (EGCG) is the most abundant and biologically active catechin polyphenol found in green tea (Camellia sinensis). EGCG exhibits potent antioxidant activity, acting as a free radical scavenger and metal ion chelator, and modulates multiple signaling pathways involved in cell proliferation, apoptosis, angiogenesis, and inflammation. It has been shown to inhibit proteasome activity, suppress NF-κB and MAPK signaling, and modulate epigenetic regulators including DNA methyltransferases and histone acetyltransferases. EGCG has been extensively investigated for its potential roles in cancer chemoprevention, metabolic disorders, cardiovascular protection, neuroprotection, and anti-microbial activity. It is widely used in cancer biology, oxidative stress research, and natural product pharmacology studies.
Product Features
1. Potent Antioxidant: Effective free radical scavenger and metal ion chelator.
2. Multi-Pathway Modulation: Inhibits NF-κB, MAPK, and proteasome activity; modulates epigenetic regulators.
3. Broad Research Utility: Investigated in cancer, metabolic, cardiovascular, and neurodegenerative research.
4. Well-Characterized Compound: One of the most extensively studied green tea polyphenols.
5. High Purity: Supplied at 99% purity for reproducible pharmacological studies.
Specifications
Size: 50 mg / 100 mg / 500 mg / 1 g
CAS Number: 989-51-5
Purity: 99%
Molecular Weight: 458.37
Molecular Formula: C22H18O11
Solubility: DMSO: ≥20 mg/mL; Water: ≥5 mg/mL; Ethanol: ≥10 mg/mL
Storage and Stability
Storage Conditions: Store at 2-8°C, protected from light and moisture, under inert atmosphere.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed. For reconstituted solutions, aliquot and store at -20°C protected from light. Avoid repeated freeze-thaw cycles.
Protocol (For Reference Only)
Important: EGCG is light-sensitive and prone to oxidation. Protect from light and moisture during handling and storage. Prepare stock solutions in anhydrous DMSO.
1. Stock Solution Preparation: Dissolve EGCG in anhydrous DMSO to prepare a concentrated stock solution (e.g., 10-50 mM). Aliquot into single-use vials and store at -20°C protected from light under inert atmosphere.
2. In Vitro Working Solution Preparation: Dilute DMSO stock solution into culture medium to achieve working concentrations typically ranging from 10-100 µM. Limit final DMSO concentration to ≤0.1%.
3. Antioxidant and Cytoprotection Assays: Pretreat cells with EGCG at 10-50 µM for 1-24 hours before exposure to oxidative stressors. Assess cell viability, ROS levels, and antioxidant enzyme activity.
4. Cancer Cell Proliferation and Apoptosis Assays: Treat cancer cells with EGCG at 20-100 µM for 24-72 hours. Assess cell viability by MTT assay and apoptosis by annexin V/PI flow cytometry or caspase activation.
5. Signaling Pathway Analysis: Following EGCG treatment, assess NF-κB, MAPK, or Akt pathway activation by Western blot using phospho-specific antibodies.
Precautions
1. Protect from light and moisture during handling and storage; EGCG is prone to oxidation.
2. Use appropriate vehicle controls in all experiments; DMSO concentration in culture medium should not exceed 0.1%.
3. EGCG exhibits pleiotropic effects; interpret results with consideration of multiple signaling pathways.
4. Prepare working solutions fresh to minimize oxidation.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What are the main biological activities of EGCG?
A1: EGCG exhibits antioxidant, anti-inflammatory, anti-proliferative, and anti-angiogenic activities, primarily mediated through modulation of NF-κB, MAPK, and PI3K/Akt pathways.
Q2: What is the recommended concentration for in vitro studies?
A2: Typical in vitro working concentrations range from 10-100 µM. Optimize for each cell type and endpoint.
Q3: How should I store EGCG?
A3: Store at 2-8°C protected from light and moisture under inert atmosphere. Reconstituted stock solutions should be aliquoted and stored at -20°C protected from light.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of conditions is recommended for specific experimental systems.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-01220164
Product Name: (-)-Epigallocatechin Gallate
Size: 50 mg / 100 mg / 500 mg / 1 g
Suggested Retail Price:
50 mg: CNY ¥618.00 / USD $61.80 / EUR �74.16 / JPY ¥11124.00
100 mg: CNY ¥742.00 / USD $74.20 / EUR �89.04 / JPY ¥13356.00
500 mg: CNY ¥1920.00 / USD $192.00 / EUR �230.40 / JPY ¥34560.00
1 g: CNY ¥3250.00 / USD $325.00 / EUR �390.00 / JPY ¥58500.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
