Recombinant Human CXCL10 / IP-10, CYT-P02778
Product Introduction
Human C-X-C Motif Chemokine Ligand 10 (CXCL10), also known as IP-10 (10 kDa interferon gamma-induced protein), is a non-ELR chemokine secreted by various cell types, such as monocytes, endothelial cells, and fibroblasts, in response to IFN-γ. CXCL10 functions via the chemokine receptor CXCR3. CXCL10 has been attributed to several roles, including chemoattraction for activated T-lymphocytes, inhibition of angiogenesis, and antitumor activity.
Product Features
1. High Purity: Greater than 95% as determined by reducing SDS-PAGE.
2. Verified Binding Activity: Immobilized Recombinant Human CXCL10 at 2 µg/mL (100 µL/well) can bind Human CXCL10 Antibody (Eldelumab, Research Grade) with an ED50 of 7.57 ng/mL (regularly tested).
3. E. coli Expression: Produced in an E. coli expression system with the target gene encoding Val22-Pro98.
4. Low Endotoxin: <1 EU/µg as determined by LAL test.
5. Lyophilized Powder: Supplied as a lyophilized powder for convenient storage and handling.
Specifications
Size: 10 µg / 50 µg
Protein Accession: P02778
Alternative Names: C-X-C Motif Chemokine 10; 10 kDa Interferon Gamma-Induced Protein; Gamma-IP10; IP-10; Small-Inducible Cytokine B10; CXCL10; INP10; SCYB10
Molecular Weight: 8.6 kDa
Apparent Molecular Weight: 13 kDa, reducing conditions
Expression Region: Val22-Pro98
Formulation: Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4
Endotoxin: <1 EU/µg (LAL test)
Purity: Greater than 95% by reducing SDS-PAGE
Binding Activity: Immobilized Recombinant Human CXCL10 at 2 µg/mL (100 µL/well) binds Human CXCL10 Antibody (Eldelumab) with an ED50 of 7.57 ng/mL
Storage and Stability
Shipping Conditions: Shipped at ambient temperature. Upon receipt, store immediately at the temperature listed below.
Storage Conditions:
- Lyophilized protein: Store at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution: Store at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples: Stable at ≤ -20°C for 3 months.
Protocol (For Reference Only)
Important: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/mL.
1. Reconstitution: Dissolve the lyophilized protein in distilled water. Allow to dissolve completely with gentle mixing; do not vortex.
2. Aliquoting: Aliquot the reconstituted solution into single-use volumes to minimize freeze-thaw cycles.
3. Storage: Store aliquots at ≤ -20°C for up to 3 months. Reconstituted solution may be stored at 2-8°C for 2-7 days.
4. Binding Assay: Immobilized Recombinant Human CXCL10 at 2 µg/mL (100 µL/well) can bind Human CXCL10 Antibody with an ED50 of 7.57 ng/mL.
5. Working Concentration: Optimal working concentrations should be determined empirically for each cell type and experimental system.
Precautions
1. Always centrifuge tubes before opening.
2. Do not mix by vortex or pipetting; gentle inversion or swirling is recommended.
3. It is not recommended to reconstitute to a concentration less than 100 µg/mL to avoid protein loss.
4. Aliquot reconstituted solution to minimize freeze-thaw cycles.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the biological function of CXCL10?
A1: CXCL10 functions via CXCR3 and mediates chemoattraction of activated T-lymphocytes, inhibition of angiogenesis, and antitumor activity.
Q2: How should I reconstitute this protein?
A2: Dissolve the lyophilized protein in distilled water to a concentration of at least 100 µg/mL. Do not vortex or pipette vigorously.
Q3: How should I store the reconstituted protein?
A3: Reconstituted solution can be stored at 2-8°C for 2-7 days. For longer storage, aliquot and store at ≤ -20°C for up to 3 months. Avoid repeated freeze-thaw cycles.
Q4: What is the endotoxin level?
A4: <1 EU/µg as determined by LAL test.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of working concentrations is recommended for specific cell types and experimental systems.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: CYT-P02778
Product Name: Recombinant Human CXCL10 / IP-10
Size: 10 µg / 50 µg
Suggested Retail Price:
10 µg: CNY ¥1640.00 / USD $164.00 / EUR �196.80 / JPY ¥29520.00
50 µg: CNY ¥6520.00 / USD $652.00 / EUR �782.40 / JPY ¥117360.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
