Organoid Cryopreservation Medium, SF1800-06
Product Introduction
Organoid Cryopreservation Medium is suitable for the long-term low-temperature storage of organoids and cell lines derived from various mammalian species, such as human and mouse.
Organoid (Cell) Cryopreservation
(1) Add pre-chilled (2-8°C) Organoid Cryopreservation Medium to the recovered organoids (cells), mix by pipetting, and immediately transfer to a cryovial (the cell suspension volume in the cryovial should be greater than 0.5 mL). Note: Each 1 mL of cryopreservation medium is recommended for 1×10³ to 1×10⁷ cells or an equivalent amount of organoids.
(2) Place the cryovial into a cell freezing container (the container must be pre-equilibrated to room temperature or 4°C), then immediately place the container in a -80°C ultra-low temperature freezer. Alternatively, perform manual gradient cooling: stand at 4°C for 10 minutes, store at -20°C for 1 hour, and store at -80°C overnight.
(3) The next day or after 12 hours, transfer the cryovial under low-temperature conditions (not higher than -70°C; dry ice or the original freezing container is recommended) to liquid nitrogen for long-term storage.
Organoid (Cell) Recovery
(1) Add 10 mL of Organoid Passaging Buffer (or PBS) to a 15 mL centrifuge tube.
(2) Remove the frozen organoids/cells from the liquid nitrogen tank and quickly place in a 37°C water bath to thaw.
(3) During thawing, gently shake the cryovial to ensure complete thawing of the cryopreservation medium within 1-2 minutes.
(4) Quickly transfer the thawed organoids to the 15 mL centrifuge tube, gently pipette 6-8 times, centrifuge at 300 x g for 5 minutes, then remove the supernatant and collect the organoid/cell pellet. Add an appropriate volume of Organoid Passaging Buffer (or PBS) to resuspend, transfer to a 1.5 mL centrifuge tube, and centrifuge at 300 x g for 5 minutes.
(5) Resuspend in 20-25 times the centrifuged organoid volume of Matrigel, seed 25 µL of Matrigel per well in a 24-well cell culture plate, place in the incubator for 10-15 minutes to solidify, and add 500-750 µL of organoid culture medium.
Product Features
1. Multi-Species Compatibility: Suitable for organoids and cell lines derived from various mammalian species, including human and mouse.
2. Optimized Formulation: Supports long-term low-temperature storage while maintaining organoid viability and function.
3. Convenient Protocol: Compatible with both controlled-rate freezing containers and manual gradient cooling.
4. Ready-to-Use: Supplied as a ready-to-use solution requiring no preparation.
Specifications
Size: 50 mL / 100 mL
Application: Cryopreservation of organoids and cell lines
Recommended Cell Density: 1×10³ to 1×10⁷ cells per 1 mL cryopreservation medium
Storage and Stability
Storage Conditions: Store at 2-8°C or -20°C according to product label. Pre-chill to 2-8°C before use.
Shelf Life: The product is stable for 12 months from the date of manufacture when stored as directed.
Protocol (For Reference Only)
Important: Pre-chill the cryopreservation medium to 2-8°C before use. Ensure the freezing container is pre-equilibrated to room temperature or 4°C before use.
1. Cryopreservation:
1.1 Add pre-chilled (2-8°C) Organoid Cryopreservation Medium to the recovered organoids (cells), mix by pipetting, and immediately transfer to a cryovial (cell suspension volume >0.5 mL). Each 1 mL of cryopreservation medium is recommended for 1×10³ to 1×10⁷ cells or an equivalent amount of organoids.
1.2 Place the cryovial into a pre-equilibrated cell freezing container, then immediately place in a -80°C freezer. Alternatively, perform manual gradient cooling: 4°C for 10 minutes, -20°C for 1 hour, -80°C overnight.
1.3 The next day or after 12 hours, transfer the cryovial under low-temperature conditions (not higher than -70°C) to liquid nitrogen for long-term storage.
2. Recovery:
2.1 Add 10 mL of Organoid Passaging Buffer (or PBS) to a 15 mL centrifuge tube.
2.2 Remove the frozen organoids/cells from liquid nitrogen and quickly place in a 37°C water bath to thaw.
2.3 Gently shake the cryovial during thawing to ensure complete thawing within 1-2 minutes.
2.4 Quickly transfer the thawed organoids to the 15 mL centrifuge tube, gently pipette 6-8 times, centrifuge at 300 x g for 5 minutes, remove supernatant, and collect the pellet. Resuspend in an appropriate volume of Organoid Passaging Buffer (or PBS), transfer to a 1.5 mL centrifuge tube, and centrifuge at 300 x g for 5 minutes.
2.5 Resuspend in 20-25 times the organoid pellet volume of Matrigel, seed 25 µL per well in a 24-well plate, allow to solidify in the incubator for 10-15 minutes, and add 500-750 µL of organoid culture medium.
Precautions
1. Pre-chill the cryopreservation medium to 2-8°C before use.
2. Ensure the freezing container is pre-equilibrated to room temperature or 4°C before use.
3. Transfer cryovials to liquid nitrogen under low-temperature conditions (not higher than -70°C) to maintain viability.
4. Avoid repeated freeze-thaw cycles of the cryopreservation medium.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What species are suitable for this cryopreservation medium?
A1: This medium is suitable for organoids and cell lines derived from various mammalian species, including human and mouse.
Q2: What is the recommended cell density for cryopreservation?
A2: Each 1 mL of cryopreservation medium is recommended for 1×10³ to 1×10⁷ cells or an equivalent amount of organoids.
Q3: Can I use manual gradient cooling instead of a freezing container?
A3: Yes. Manual gradient cooling (4°C for 10 minutes, -20°C for 1 hour, -80°C overnight) may be used as an alternative to a controlled-rate freezing container.
Q4: How should I store the cryopreservation medium?
A4: Store at 2-8°C or -20°C according to product label. Pre-chill to 2-8°C before use. Avoid repeated freeze-thaw cycles.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of cryopreservation and recovery conditions is recommended for specific organoid types.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: SF1800-06
Product Name: Organoid Cryopreservation Medium
Size: 50 mL / 100 mL
Suggested Retail Price:
50 mL: CNY ¥950.00 / USD $95.00 / EUR �114.00 / JPY ¥17100.00
100 mL: CNY ¥1460.00 / USD $146.00 / EUR �175.20 / JPY ¥26280.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
