Goat Anti-Mouse IgG1-AF594, SA-59401-1
Product Introduction
This product is a goat polyclonal secondary antibody conjugated to AcelFluor 594 (AF594), specifically recognizing the heavy chain of mouse IgG1. Secondary antibodies are essential reagents in immunofluorescence (IF), immunohistochemistry (IH), flow cytometry (FC), ELISA (E), and other immunodetection techniques, providing specific detection of primary antibodies bound to target antigens. AF594 is a bright, photostable orange-red fluorescent dye with excitation/emission maxima of approximately 590/617 nm, compatible with standard TRITC/Texas Red filter sets and 561 nm laser-equipped instruments. The antibody is isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads, ensuring high specificity and minimal cross-reactivity. By immunoelectrophoresis and ELISA, this antibody reacts specifically with mouse IgG1 heavy chain.
Product Features
1. AF594 Conjugate: Bright orange-red fluorescence with Ex/Em of 590/617 nm.
2. Subclass-Specific: Specifically recognizes mouse IgG1 heavy chain with minimal cross-reactivity.
3. Immunoaffinity Purified: Isolated by immunoaffinity chromatography for high specificity.
4. Broad Application: Validated for IH, IF, FC, and ELISA.
5. Ready-to-Use Liquid Format: Supplied in stabilized buffer with BSA and glycerol for convenient handling.
Specifications
Size: 100 µg (100 µL) / 500 µg (500 µL) / 1 mg (1 mL)
Host Species: Goat
Target Species: Mouse
Immunogen: Mouse IgG1
Specificity: Mouse IgG1 heavy chain (verified by immunoelectrophoresis and ELISA)
Clonality: Polyclonal
Conjugate: AcelFluor 594 (AF594)
Concentration: 1 mg/mL
Excitation/Emission: 590 nm / 617 nm
Form: Liquid in 0.01 M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide
Recommended Dilution: IH (1:100 - 1:1,000), IF (1:100 - 1:1,000), FC (1:1,000 - 1:4,000), ELISA (assay dependent)
Storage and Stability
Storage Conditions: Shipped at 4¡ãC. Upon delivery, aliquot and store at -20¡ãC for one year. Avoid freeze/thaw cycles.
Shelf Life: The product is stable for 12 months from the date of delivery when stored as directed.
Protocol (For Reference Only)
Important: Protect fluorescent conjugate from light. Centrifuge the vial briefly before opening. Optimal working dilution should be determined empirically for each application.
1. Immunofluorescence (IF): Fix and permeabilize samples according to standard protocols. Block with appropriate blocking buffer. Incubate with mouse IgG1 primary antibody. Wash and incubate with AF594-conjugated secondary antibody at 1:100 to 1:1,000 dilution for 1 hour at room temperature in the dark. Wash and mount with anti-fade mounting medium.
2. Immunohistochemistry (IH): Process tissue sections according to standard protocols. Following mouse IgG1 primary antibody incubation and washing, apply AF594-conjugated secondary antibody at 1:100 to 1:1,000 dilution for 30-60 minutes at room temperature in the dark. Wash and mount.
3. Flow Cytometry (FC): Following cell staining with mouse IgG1 primary antibody, wash cells and incubate with AF594-conjugated secondary antibody at 1:1,000 to 1:4,000 dilution for 30 minutes at 4¡ãC in the dark. Wash and analyze using a 561 nm laser with appropriate emission filter.
4. ELISA (E): Dilute the AF594-conjugated secondary antibody according to assay requirements and incubate following primary antibody binding. Detect according to the specific ELISA format.
Precautions
1. Protect from light to prevent photobleaching of the AF594 fluorophore.
2. Avoid freeze/thaw cycles; aliquot upon delivery and store at -20¡ãC.
3. The recommended dilutions are guidelines; optimal dilution must be determined empirically for each application.
4. Sodium azide is included as a preservative; handle with appropriate precautions.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the excitation/emission of AF594?
A1: AF594 has excitation/emission maxima of approximately 590/617 nm, compatible with TRITC/Texas Red filter sets and 561 nm lasers.
Q2: What is the specificity of this antibody?
A2: This antibody specifically recognizes mouse IgG1 heavy chain, as verified by immunoelectrophoresis and ELISA.
Q3: How should I store this product?
A3: Upon delivery, aliquot and store at -20¡ãC protected from light. Avoid repeated freeze/thaw cycles.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of antibody dilution and staining conditions is recommended.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: SA-59401-1
Product Name: Goat Anti-Mouse IgG1 - AF594
Size: 100 µg (100 µL) / 500 µg (500 µL) / 1 mg (1 mL)
Suggested Retail Price:
100 µg (100 µL): CNY ¥2680.00 / USD $268.00 / EUR €321.60 / JPY ¥48240.00
500 µg (500 µL): CNY ¥4850.00 / USD $485.00 / EUR €582.00 / JPY ¥87300.00
1 mg (1 mL): CNY ¥8500.00 / USD $850.00 / EUR €1020.00 / JPY ¥153000.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
