Goat Anti-Mouse IgG2a-AF647, SA-64701-2A
Product Introduction
This product is a goat polyclonal secondary antibody conjugated to AcelFluor 647 (AF647), specifically recognizing the heavy chain of mouse IgG2a. Secondary antibodies are essential reagents in immunofluorescence (IF), immunohistochemistry (IH), flow cytometry (FC), ELISA (E), and other immunodetection techniques, providing specific detection of primary antibodies bound to target antigens. AF647 is a bright, photostable far-red fluorescent dye with excitation/emission maxima of approximately 650/665 nm, compatible with standard Cy5/APC filter sets and 633-640 nm laser-equipped instruments. The antibody is isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads, ensuring high specificity and minimal cross-reactivity. By immunoelectrophoresis and ELISA, this antibody reacts specifically with mouse IgG2a heavy chain.
Product Features
1. AF647 Conjugate: Bright far-red fluorescence with Ex/Em of 650/665 nm.
2. Subclass-Specific: Specifically recognizes mouse IgG2a heavy chain with minimal cross-reactivity.
3. Immunoaffinity Purified: Isolated by immunoaffinity chromatography for high specificity.
4. Broad Application: Validated for IH, IF, FC, and ELISA.
5. Ready-to-Use Liquid Format: Supplied in stabilized buffer with BSA and glycerol for convenient handling.
Specifications
Size: 100 µg (100 µL) / 500 µg (500 µL) / 1 mg (1 mL)
Host Species: Goat
Target Species: Mouse
Immunogen: Mouse IgG2a
Specificity: Mouse IgG2a heavy chain (verified by immunoelectrophoresis and ELISA)
Clonality: Polyclonal
Conjugate: AcelFluor 647 (AF647)
Concentration: 1 mg/mL
Excitation/Emission: 650 nm / 665 nm
Form: Liquid in 0.01 M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide
Recommended Dilution: IH (1:100 - 1:1,000), IF (1:100 - 1:1,000), FC (1:1,000 - 1:4,000), ELISA (assay dependent)
Storage and Stability
Storage Conditions: Shipped at 4¡ãC. Upon delivery, aliquot and store at -20¡ãC for one year. Avoid freeze/thaw cycles.
Shelf Life: The product is stable for 12 months from the date of delivery when stored as directed.
Protocol (For Reference Only)
Important: Protect fluorescent conjugate from light. Centrifuge the vial briefly before opening. Optimal working dilution should be determined empirically for each application.
1. Immunofluorescence (IF): Fix and permeabilize samples according to standard protocols. Block with appropriate blocking buffer. Incubate with mouse IgG2a primary antibody. Wash and incubate with AF647-conjugated secondary antibody at 1:100 to 1:1,000 dilution for 1 hour at room temperature in the dark. Wash and mount with anti-fade mounting medium.
2. Immunohistochemistry (IH): Process tissue sections according to standard protocols. Following mouse IgG2a primary antibody incubation and washing, apply AF647-conjugated secondary antibody at 1:100 to 1:1,000 dilution for 30-60 minutes at room temperature in the dark. Wash and mount.
3. Flow Cytometry (FC): Following cell staining with mouse IgG2a primary antibody, wash cells and incubate with AF647-conjugated secondary antibody at 1:1,000 to 1:4,000 dilution for 30 minutes at 4¡ãC in the dark. Wash and analyze using a 633-640 nm laser with appropriate emission filter.
4. ELISA (E): Dilute the AF647-conjugated secondary antibody according to assay requirements and incubate following primary antibody binding. Detect according to the specific ELISA format.
Precautions
1. Protect from light to prevent photobleaching of the AF647 fluorophore.
2. Avoid freeze/thaw cycles; aliquot upon delivery and store at -20¡ãC.
3. The recommended dilutions are guidelines; optimal dilution must be determined empirically for each application.
4. Sodium azide is included as a preservative; handle with appropriate precautions.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the excitation/emission of AF647?
A1: AF647 has excitation/emission maxima of approximately 650/665 nm, compatible with Cy5/APC filter sets and 633-640 nm lasers.
Q2: What is the specificity of this antibody?
A2: This antibody specifically recognizes mouse IgG2a heavy chain, as verified by immunoelectrophoresis and ELISA.
Q3: How should I store this product?
A3: Upon delivery, aliquot and store at -20¡ãC protected from light. Avoid repeated freeze/thaw cycles.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of antibody dilution and staining conditions is recommended.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: SA-64701-2A
Product Name: Goat Anti-Mouse IgG2a - AF647
Size: 100 µg (100 µL) / 500 µg (500 µL) / 1 mg (1 mL)
Suggested Retail Price:
100 µg (100 µL): CNY ¥2680.00 / USD $268.00 / EUR €321.60 / JPY ¥48240.00
500 µg (500 µL): CNY ¥4850.00 / USD $485.00 / EUR €582.00 / JPY ¥87300.00
1 mg (1 mL): CNY ¥8500.00 / USD $850.00 / EUR €1020.00 / JPY ¥153000.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
