Dipeptidyl Peptidase IV (DPP4) Inhibitor Activity Fluorometric Assay Kit, IC-6577

Product Introduction

Dipeptidyl peptidase 4 (DPP4, EC 3.4.14.5), also known as CD26, is a type II transmembrane serine protease widely distributed on the surface of human cells. It cleaves peptide bonds at the second proline or alanine residue from the N-terminus of peptide chains. In vivo, DPP4 rapidly degrades incretins that promote blood glucose reduction. DPP4 inhibitors are a class of oral antidiabetic drugs that regulate blood glucose by inhibiting DPP4 activity. DPP4 cleaves its substrate to release the fluorescent compound AMC. This kit measures the change in the rate of fluorescence increase to calculate the inhibitory activity of DPP4 inhibitors.

Product Features

1. Fluorescent AMC Method: Measures DPP4 inhibitor activity based on the release of fluorescent AMC from the substrate.

2. Rapid Assay: Estimated assay time of approximately 40 minutes.

3. Broad Sample Compatibility: Suitable for animal tissues and serum/plasma samples.

4. Convenient Format: Available in 50T/48S specification.

Specifications

Size: 50T/48S

Detection Method: Fluorometric

Detection Wavelength: Ex 360 nm, Em 460 nm

Detection Instrument: Fluorescence microplate reader or multifunctional microplate reader with fluorescence detection capability

Estimated Assay Time: 40 minutes

Response Type: Decreasing type

Sample Types: Animal tissues; serum (plasma)

Research Fields: Enzymology; Biochemistry; Pharmacology

Storage and Stability

Storage Conditions: Store at -20¡ãC for 6 months.

Shipping Conditions: Frozen transport.

Shelf Life: 6 months from the date of manufacture when stored at -20¡ãC as directed.

Protocol (For Reference Only)

Important: This product is for research use only. Equilibrate reagents to the appropriate temperature before use. Prepare working solutions fresh according to the kit instructions.

1. Sample Preparation: Prepare animal tissue homogenates or serum/plasma samples according to standard protocols. Centrifuge to remove debris and collect the supernatant for analysis.

2. Reagent Preparation: Prepare the reaction working solution according to the kit instructions. Equilibrate reagents to the assay temperature before use.

3. Reaction Setup: Add samples, controls, and reaction working solution to the designated wells of a black 96-well plate according to the kit instructions.

4. Detection: Measure fluorescence at Ex 360 nm/Em 460 nm using a fluorescence microplate reader. Record fluorescence values at appropriate time intervals.

5. Calculation: Calculate the rate of fluorescence increase and determine DPP4 inhibitor activity according to the kit instructions.

Precautions

1. This product is for research use only. Not for use in medical, clinical diagnostic, or therapeutic applications, food, or cosmetics. Do not store in ordinary residential areas.

2. Wear laboratory protective clothing, disposable gloves, and a mask during operation.

3. Experimental results may be affected by various factors; related handling is limited to the product itself and does not involve other compensation.

4. For research use only. Not for use in diagnostic or therapeutic procedures.

FAQ (Simplified)

Q1: What is the detection principle of this kit?

A1: DPP4 cleaves its substrate to release the fluorescent compound AMC. The kit measures the change in the rate of fluorescence increase to calculate the inhibitory activity of DPP4 inhibitors.

Q2: What detection instrument is required?

A2: A fluorescence microplate reader or multifunctional microplate reader with fluorescence detection capability is required, with detection at Ex 360 nm/Em 460 nm.

Q3: What sample types are suitable for this kit?

A3: This kit is suitable for animal tissues and serum/plasma samples.

Q4: How should I store this kit?

A4: Store at -20¡ãC for 6 months. Shipped under frozen conditions.

Disclaimer

1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.

2. Due to the variable nature of biological research, optimization of sample preparation and assay conditions is recommended for specific sample types.

3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.

4. Wear appropriate protective clothing and gloves when handling this product.

Ordering Information

Catalog Number: IC-6577

Product Name: Dipeptidyl Peptidase IV (DPP4) Inhibitor Activity Fluorometric Assay Kit

Size: 50T/48S

Suggested Retail Price: CNY ¥1950.00 / USD $195.00 / EUR €234.00 / JPY ¥35100.00

Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.