BODIPY 558/568 C12, 158757-84-7, IC-03718039
Product Introduction
BODIPY 558/568 C12 is a lipophilic fluorescent fatty acid analog consisting of the BODIPY 558/568 fluorophore conjugated to a 12-carbon (C12) fatty acid chain. BODIPY dyes exhibit high fluorescence quantum yield, narrow emission bandwidths, and excellent photostability compared with conventional fluorophores. The C12 fatty acid chain enables the probe to partition into lipid membranes and associate with lipid droplets, making it suitable for studying lipid uptake, trafficking, storage, and metabolism in living cells. BODIPY 558/568 C12 exhibits excitation/emission maxima of approximately 558/568 nm, which is compatible with TRITC/Texas Red filter sets and 561 nm laser-equipped instruments, and is well-suited for multi-color imaging applications.
Product Features
1. Lipophilic Fatty Acid Analog: C12 chain enables partitioning into lipid membranes and association with lipid droplets.
2. Bright and Photostable: BODIPY fluorophore offers high quantum yield, narrow emission bandwidths, and excellent photostability.
3. Multi-Color Compatible: Emission at ~568 nm is compatible with TRITC/Texas Red filter sets and well-separated from green and far-red fluorophores.
4. Live-Cell Compatible: Suitable for live-cell imaging of lipid uptake, trafficking, storage, and metabolism.
5. High Purity: Supplied at 98% purity for reproducible staining.
Specifications
Size: 1 mg / 5 mg
CAS Number: 158757-84-7
Purity: 98%
Molecular Weight: 668.43
Molecular Formula: C34H51BF2N4O2
Excitation/Emission: ~558 nm / ~568 nm
Solubility: DMSO: ¡Ý10 mg/mL; Ethanol: ¡Ý5 mg/mL; limited solubility in aqueous buffers
Storage and Stability
Storage Conditions: Store at -20¡ãC under inert atmosphere, protected from light and moisture.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed. For reconstituted solutions, aliquot and store at -20¡ãC protected from light. Avoid repeated freeze-thaw cycles.
Protocol (For Reference Only)
Important: Protect from light during handling and storage. Prepare stock solutions in anhydrous DMSO. For live-cell imaging, prepare working solutions fresh before use.
1. Stock Solution Preparation: Dissolve BODIPY 558/568 C12 in anhydrous DMSO to prepare a concentrated stock solution (e.g., 1-10 mM). Aliquot into single-use vials and store at -20¡ãC protected from light under inert atmosphere.
2. Working Solution Preparation: Dilute the DMSO stock solution into serum-free culture medium or assay buffer to achieve working concentrations typically ranging from 0.1-10 µM. Limit final DMSO concentration in culture medium to ¡Ü0.1%.
3. Cell Labeling: Incubate cells with BODIPY 558/568 C12 working solution at 37¡ãC for 10-30 minutes, or as optimized for the specific application. For lipid uptake studies, co-incubate with unlabeled fatty acids or inhibitors as required.
4. Washing: Remove the labeling solution and wash cells 2-3 times with PBS or serum-free medium to remove unincorporated probe.
5. Detection: Visualize by fluorescence microscopy using a TRITC/Texas Red filter set or analyze by flow cytometry using an appropriate laser and emission filter.
Precautions
1. Protect from light and moisture during handling and storage.
2. Prepare stock solutions in anhydrous DMSO and aliquot into single-use volumes.
3. Use appropriate vehicle controls; DMSO concentration in culture medium should not exceed 0.1%.
4. Optimize labeling concentration and incubation time for each cell type and application.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is BODIPY 558/568 C12 used for?
A1: It is a lipophilic fluorescent fatty acid analog used for studying lipid uptake, trafficking, storage, and metabolism in living cells, including lipid droplet labeling.
Q2: What are the excitation/emission wavelengths?
A2: BODIPY 558/568 C12 has excitation/emission maxima of approximately 558/568 nm, compatible with TRITC/Texas Red filter sets.
Q3: How should I prepare the working solution?
A3: Dissolve in anhydrous DMSO to prepare a stock solution, then dilute into serum-free medium or assay buffer to a working concentration of 0.1-10 µM.
Q4: How should I store this product?
A4: Store at -20¡ãC under inert atmosphere, protected from light and moisture. Aliquot stock solutions into single-use volumes.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of labeling conditions is recommended for specific cell types.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-03718039
Product Name: BODIPY 558/568 C12
Size: 1 mg / 5 mg
Suggested Retail Price:
1 mg: CNY ¥1650.00 / USD $165.00 / EUR €198.00 / JPY ¥29700.00
5 mg: CNY ¥6500.00 / USD $650.00 / EUR €780.00 / JPY ¥117000.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
