Biotin-PEG1000-NHS, IC-178901K
Product Introduction
Biotin-PEG-NHS is a heterobifunctional PEG derivative containing a biotin group and an N-hydroxysuccinimide (NHS) active ester. The PEG chain confers water solubility, reduced immunogenicity, and reduced non-specific binding, while the two functional termini enable orthogonal bioconjugation. The NHS ester reacts readily with primary amines (e.g., lysine residues and N-terminal amines) to form stable amide bonds, while biotin exhibits high-affinity binding to streptavidin and avidin. This dual functionality makes Biotin-PEG-NHS widely used for the biotinylation of proteins, antibodies, peptides, and other amine-containing biomolecules, and for applications in immunoassays, affinity purification, detection systems, and surface functionalization. The biotin-avidin system is widely employed for qualitative and quantitative detection of trace antigens and antibodies.
Product Features
1. Heterobifunctional Design: NHS ester for amine coupling and biotin for streptavidin/avidin binding.
2. PEG Linker: Improves water solubility, reduces steric hindrance, and minimizes non-specific binding.
3. Stable Amide Linkage: NHS ester forms stable amide bonds with primary amines.
4. High End-Group Substitution: Biotin ≥90%, NHS ≥90%.
5. Broad Application: Suitable for protein and antibody biotinylation, immunoassays, affinity purification, and surface modification.
Specifications
Size: 500 mg / 1 g / 5 g
Catalog Number: IC-178901K
English Name: Biotin-PEG-N-Hydroxy succinimide
PEG Molecular Weight: 1,000 (1K)
Purity: 90+
End-Group Substitution: Biotin ≥90%, NHS ≥90%
Appearance: Paste to powder (depending on molecular weight)
Solubility: Water (not recommended), dichloromethane, DMSO
Storage and Stability
Storage Conditions: Store dry at -20°C.
Shelf Life: One year, with re-testing recommended after one year.
Handling: Remove the product from the refrigerator and allow it to gradually warm to room temperature in a desiccator before opening the cap. After use, fill the container with inert gas, seal with parafilm, tighten the cap, place in a zip-lock bag, and return to the refrigerator promptly. Prepare solutions fresh immediately before use.
Protocol (For Reference Only)
Important: Biotin-PEG-NHS contains an NHS group and is moisture-sensitive. The active ester hydrolyzes upon contact with water. Use anhydrous solvents and avoid moisture during handling and storage.
1. Stock Solution Preparation: Dissolve Biotin-PEG-NHS in anhydrous DMSO or dichloromethane to prepare a concentrated stock solution immediately before use. Do not use water as a solvent.
2. Protein Biotinylation: Dissolve the protein to be biotinylated in amine-free buffer (e.g., PBS or 0.1 M sodium bicarbonate buffer, pH 7.5-8.5) at a concentration of 1-10 mg/mL. Add Biotin-PEG-NHS stock solution at a molar ratio of 5-20:1 (Biotin-PEG-NHS:protein). Incubate at room temperature for 30-60 minutes or at 4°C overnight with gentle mixing.
3. Purification: Remove unreacted Biotin-PEG-NHS by gel filtration chromatography, dialysis, or ultrafiltration.
4. Verification: Confirm biotinylation by streptavidin-HRP binding assay, HABA assay, or mass spectrometry.
5. Storage of Conjugates: Store purified conjugates at 4°C for short-term use or aliquot and store at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
Precautions
1. Biotin-PEG-NHS is moisture-sensitive; the NHS ester hydrolyzes upon contact with water. Use anhydrous solvents and avoid moisture during handling.
2. Allow the product to warm to room temperature in a desiccator before opening to prevent condensation.
3. After use, fill the container with inert gas, seal tightly, and store at -20°C under dry conditions.
4. Avoid buffers containing primary amines (e.g., Tris, glycine) during conjugation reactions.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the reactive group of Biotin-PEG-NHS?
A1: The NHS ester reacts with primary amines on proteins and other biomolecules to form stable amide bonds, while the biotin group binds with high affinity to streptavidin and avidin.
Q2: What solvents should I use?
A2: Use anhydrous DMSO or dichloromethane. Water is not recommended because the NHS ester hydrolyzes in aqueous solutions.
Q3: How should I store Biotin-PEG-NHS?
A3: Store dry at -20°C. Allow to warm to room temperature in a desiccator before opening, and after use fill with inert gas and seal tightly.
Q4: What is the PEG molecular weight of this product?
A4: The PEG molecular weight is 1,000 (1K).
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of bioconjugation reactions, optimization of molar ratios and reaction conditions is recommended for specific biomolecules.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-178901K
Product Name: Biotin-PEG-NHS
Size: 500 mg / 1 g / 5 g
Suggested Retail Price:
500 mg: CNY ¥1500.00 / USD $150.00 / EUR �180.00 / JPY ¥27000.00
1 g: CNY ¥2560.00 / USD $256.00 / EUR �307.20 / JPY ¥46080.00
5 g: CNY ¥9500.00 / USD $950.00 / EUR �1140.00 / JPY ¥171000.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
