CellPro MEM NEAA,100x (IC-1321)

Non-Essential Amino Acids Solution (NEAA)

Description

CellPro MEM NEAA(Non-Essential Amino Acids) , 100x is a sterile-filtered, concentrated (100x) solution containing a defined mixture of amino acids classified as non-essential for mammalian cell cultures. These amino acids are not strictly required for cell survival but are crucial for optimal growth, proliferation, and specific protein expression in many cell lines. Supplementing basal media with this solution helps reduce metabolic stress on cells, minimizes the consumption of essential amino acids, and can improve cell viability and density, particularly in long-term cultures, stressed cells, or in defined media systems.

Composition

Formulation: Based on the standard MEM (Minimum Essential Medium) formulation of non-essential amino acids.

Concentration: 100-fold concentrated solution.

Typical Amino Acids & Concentration (per liter of 1x working solution):

L-Alanine: 8.9 mg

L-Asparagine (anhydrous): 13.2 mg

L-Aspartic Acid: 13.3 mg

L-Glutamic Acid: 14.7 mg

Glycine: 7.5 mg

L-Proline: 11.5 mg

L-Serine: 10.5 mg

Solvent: Prepared in 0.85% NaCl solution.

pH: Adjusted to approximately 7.0.

Osmolality: ~290 mOsm/kg (for 1x final concentration in media).

Physical Form

Clear, colorless to slightly yellowish sterile liquid solution.

Storage and Stability

Store at 2¡ãC to 8¡ãC in the dark. DO NOT FREEZE.

The product is stable until the expiration date printed on the label when stored under recommended conditions.

After opening, ensure the bottle is tightly closed to maintain sterility. It is recommended to use aseptically and store any remaining solution under recommended conditions.

For long-term use, aliquoting is recommended to minimize contamination risk.

Directions for Use

1.  Aseptically add the solution to your sterile basal cell culture medium (e.g., DMEM, MEM, RPMI-1640) under a laminar flow hood.

2.  Standard Supplementation: Add 10 mL of CellPro MEM NEAA (100x) to 990 mL of medium to achieve a 1x final concentration. (Final dilution: 1:100).

3.  Mix the complete medium gently but thoroughly.

4.  The complete medium supplemented with NEAA should be stored at 2¡ãC to 8¡ãC and used within the stability period of the basal medium.

Quality Control

Each batch is tested for sterility .

Each batch is tested for endotoxin level. The endotoxin level is typically <1.0 EU/mL (for the 1x working concentration in culture medium).

Functionality tested in representative mammalian cell lines (e.g., HEK 293, CHO-K1) for support of cell growth.

Precautions and Warnings

For research use only. Not intended for human or therapeutic use.

Use personal protective equipment (lab coat, gloves, safety glasses).

This product is manufactured under sterile conditions. However, all handling should be performed using aseptic technique to prevent microbial contamination.

For disposal, consult local, regional, and national regulations regarding chemical and biological waste.

Manufactured by:CellPro Laboratories

For Research Use Only. Not for use in diagnostic procedures.