Mounting Medium,Antifading

Cata.No.:IC-20016

Description of Mounting Media

-Mounting Media are unsurpassed in preventing photobleaching. The different formulations of -Mounting Media all offer the same outstanding anti-fade and anti-photobleaching properties. 

-They are all compatible with fluorescein, Texas Red, AMCA, DyLight; dyes, Alexa Fluor; dyes, fluorescent nuclear stains, fluorescent proteins, fluorescent tracers,histochemical stains, and most fluorochromes.
Features of  Mounting Media
-Inhibit photobleaching of most fluorochromes 
-Available in non-hardening or hardening formulations 
-Available with or without DAPI or propidium iodide 
-No warming necessary 
-Slides can be viewed after prolonged storage 
-Continues to inhibit photobleaching after prolonged storage of mounted slides 
-Easy to use 
-Optically clear 
-Ideal refractive index

The original Mounting Medium is a glycerol-based, aqueous mountant that does not solidify but remains a viscous liquid on the slide. After mounting, coverslipped slides will not readily dry out and can be reviewed for weeks afterwards without sealing. For prolonged storage, coverslips can be permanently sealed around the perimeter with nail polish. Mounted slides should be stored at 4¡æ.

Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (located in product information box) to determine if Mounting Media  will be compatible in your system.

Product BackGround
Mounting Medium,Antifading is a kind of sealing agent used to slow down fluorescence decay. Based on glycerin and adding anti fluorescence attenuator, it has strong anti fluorescence attenuating effect and is used for sealing fluorescent tissue and cell samples. After sealing, the sample can be stored for 2-3 weeks at 4 ¡æ or - 20 ¡æ in dark. The reagent is easy to operate. When sealing, drop a drop of anti fluorescence attenuation sealing solution with a pipette gun and cover the cover glass.
Usage 
Adherent cell sample
A. After dyeing, suck up the liquid.
B. Drop a drop of Mounting Medium,Antifading on the slide, cover the cover slide with cells, let the cells contact the sealing solution, and try to avoid bubbles.
C. The sample can then be observed with a fluorescence microscope.
Tissue section
A. After dyeing, suck up the liquid.
B. Drop a drop of Mounting Medium,Antifading on the tissue slice, cover the cover glass, let the slice contact the sealing liquid, and try to avoid bubbles.
C. The sample can then be observed with a fluorescence microscope.
3. Other samples: other samples refer to cell samples or tissue sections for operation.

Please note:  Mounting Medium may not be compatible with all enzymatic substrates or fluorescent proteins and test sections would be advisable when using anything other than commonly used fluorochromes.

Refractive Index for Mounting Medium 

The refractive index for Mounting Medium is 1.44.

Reference 

[1]R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.

Order Information

Cat./REF.	Size	Price($£©	Price(€)	Price(£¤/CNY£©	Price(£¤/JYP£©
IC-20016	5ml	$36.25	€ 43.50	£¤362.50	£¤7,213.75
IC-20016	25ml	$161.25	€ 193.50	£¤1,612.50	£¤32,088.75
IC-20016	100ml	$450.00	€ 540.00	£¤4,500.00	£¤89,550.00