RNA Sta-Storage Solution£¨IC-024086£©

Description

RNA Sta-Storage Solution stabilizes and protects cellular RNA in intact, unfrozen tissue samples, eliminating the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing. Tissue pieces can be harvested and submerged in RNA Sta-Storage Solution for storage without jeopardizing the quality or quantity of RNA obtained after subsequent RNA isolation. Advantages of using RNA Sta-Storage Solution:

-Effectiveness£ºStabilize RNA for 1 day at 37¡ãC, 1 week at 25¡ãC, 1 month at 4¡ãC, or indefinitely at -20¡ãC

-Simplicity£ºA single reagent that immediately inactivates RNases and stabilizes RNA within tissues or cells

-Convenience£ºNo need to freeze samples in liquid nitrogen or rush samples back to the lab freezer

-Mobility£ºPerfect for tissue collection "in the field"

-Versatility£ºCompatible with many RNA isolation procedures, including most RNA isolation kits

Applications

RNA Sta-Storage Solution has been tested on a variety of mammalian tissues, plants, E. coli, Xenopus, fish, and Drosophila. It is ideal for:

-Protecting RNA integrity in tissues rich in RNases

-Collecting samples from different time points without having to process the samples from each time point immediately

-Archiving tissues for future microdissection

-Submerging animal cavities or organs to stabilize RNA during lengthy dissection procedures

-Collecting samples at locations (e.g., hospitals, field sites, the space shuttle) where immediate RNA isolation is not possible

-Shipping samples on wet ice or even at room temperature if shipped overnight

RNA Sta-Storage Solution procedure

The dissected tissue (less than 0.5 cm in any one dimension) is simply submerged in approximately 5 volumes of solution at room temperature. The solution permeates the cells, stabilizing the RNA.

The sample can then be stored indefinitely at -20¡ãC (the tissue does not freeze), at 4¡ãC for up to a month, or at 25¡ãC for up to a week.

For RNA isolation, the tissue is simply removed from solution and treated as though it had just been harvested. Most tissues can be transferred directly to a lysis buffer and homogenized. Samples treated with solution and then frozen can be ground with mortar and pestle or thawed and processed like fresh tissue without concern for cell rupture and release of RNases since the RNases have already been inactivated. Cells can be spun out and then added to lysis buffer, or in some cases, solution can be added along with the cells directly to the lysis buffer.

Specifications