NCI-H716(ICGE-NCI-H716,IC-82497)
Catalog No.
Size
Price
IC-82497
10^6
$498.00
IC-82497
10^6
�547.80
Overview
| Permits and Restrictions | |
|---|---|
| Organism | Homo sapiens, human |
| Tissue | cecum |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | suspension, multicell aggregates and some adherent cells |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | colorectal adenocarcinoma |
| Age | 33 years |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Karyotype |
modal number = 61; range = 55 to 64. This is a hypotriploid human cell line. The modal chromosome number is 61, occurring in 28% of cells. The rate of higher ploidies is 1.2%. Twelve marker chromosomes are common in these cells. These include t(7q,16q), t(5p,7p), t(4q,9q) and 9 others. A large number of DM''''''''s is found in every cell. The N21 is absent; and N9, N11, N13, N20, N22 and X are single-copied. No Y chromosome was detected in the Q-banded slide preparation. |
|---|---|
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| Derivation |
This line was derived from cells present in ascites fluid obtained from a patient after treatment with 5-fluorouracil.
|
| Clinical Data |
33 years
Caucasian
male
|
| Tumorigenic | Yes |
| Effects |
Yes, in nude mice
(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
|
| Comments |
The cells contain Dopa decarboxylase and, unlike other colorectal lines, contain cytoplasmic dense core granules characteristic of endocrine secretion. The cells do not express the TAG-72 or CA19-9 antigens nor do they produce carcinoembryonic antigen (CEA). |
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. |
|---|---|
| Subculturing |
Cultures can be maintained by the addition of fresh medium or replacement of medium Cultures can be established by centrifugation with subsequent resuspension at 4 to 8 x 105viable cells/mL.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
|
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
|
