T2 (174xcem.T2 )(ICGE-T2 (174xcem.T2 ),IC-84657)
Catalog No.
Size
Price
IC-84657
10^6
$498.00
IC-84657
10^6
�547.80
Overview
| Organism | Homo sapiens, human |
|---|---|
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level |
2 [Cells contain Epstein-Barr virus viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
This cell line is a suitable transfection host. Together the T1 and T2 lines are useful for studying antigen processing and T cell recognition of class I MHC antigens. |
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Derivation |
This line is a variant of the T1 cell line (ATCC CRL-1991)produced by selection the SFR1-MI.3 monoclonal antibody (against a monomorphic determinant on HLA DR).
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|---|---|
| Antigen Expression |
HLA A2; CD7 +
|
| Comments |
The cells do not express HLA DR and are Class II major histocompatibility (MHC) antigen negative.
The cells synthesize, but do not express, HLA B5.
T2 appears to have lost both copies of the CEMR.3 derived chromosome 6. The cells do not express HLA DR and are Class II major histocompatibility (MHC) antigen negative. The cells synthesize, but do not express, HLA B5.
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove''s Modified Dulbecco''s Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. |
|---|---|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 105 viable cells/mL and maintain cultures between 3 X 105 and 1 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
|
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
|
| Culture Conditions |
Temperature: 37°C
|
