A172(ICGE-A172,IC-41365)
Catalog No.
Size
Price
IC-41365
10^6
$512.00
IC-41365
10^6
�563.20
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue |
brain
|
| Product Format | frozen |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | glioblastoma |
| Age | 53 years |
| Gender | male |
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Clinical Data |
male
|
|---|---|
| Tumorigenic | No |
| Effects |
Yes, form colonies in semisolid medium.
No, the cells were not tumorigenic in immunosuppressed mice
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco''s Modified Eagle''s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
|
| Cryopreservation |
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
|
| Culture Conditions |
Temperature: 37°C
|
