

Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue | urinary bladder |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | grade 3, carcinoma |
| Age | 58 years |
| Gender | female |
| Ethnicity | Caucasian |
| Applications | This cell line is a suitable transfection host. |
Properties
| Karyotype | modal chromosome number = 104 to 121 |
|---|---|
| Clinical Data |
58 years
Caucasian
female
|
| Genes Expressed |
fibrinolytic activity; interferon
|
| Cellular Products |
fibrinolytic activity; interferon
|
| Tumorigenic | Yes |
| Effects |
Yes, in mice and hamsters
|
| Comments |
The line does not contain detectable virus particles, herpesvirus antigens or retrovirus antigens.
The cells will grow in soft agar.
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow culture to sit at room temperature or 37°C until cells detach (about 10 minutes), add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
|


