Human Cell Lines-Cell Lines-InCellGene LLC.-CellLines,Biomedicine reasearch Product,Cell Biology,Cell Culture,293T Cell,Media

Organism	Homo sapiens, human
Tissue	ureter, uroepithelium
Cell Type	epithelial SV40 immortalized
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	2 [Cells contain Adenovirus] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age	11 years
Gender	male
Storage Conditions	liquid nitrogen vapor phase
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Karyotype	The chromosome number distribution is bimodal with 50% of the cells near diploid (mode = 44 chromosomes) and 50% tetraploid (mode = 88). Both populations consistently showed seven marker chromosomes: 5p+, del(6)(p11), 9q+, 11p+, 15q-, 19p+ and Xp+. Most cells lacked a normal chromosome 15. Cells in the tetraploid population did not exactly duplicate the diploid population in that most had a 14q/21 translocation involving two chromosomes 14 and two chromosomes 21, while the diploid cells had normal chromosomes 14 and 21 and a 6q/14q translocation.
Derivation	This line was established by transformation of normal ureter tissue with SV40 virus. A chemically transformed tumorigenic derivative of this cell line is available (MC-SV-HUC T-2, see ATCC CRL-9519).
Clinical Data	male 11 years
Genes Expressed	uroepithelial keratins; SV40 T antigen
Cellular Products	uroepithelial keratins; SV40 T antigen
Tumorigenic	No
Effects	No, nude mice
Comments	The line has been repeatedly tested for production of infectious SV40 using an African Green Monkey kidney cell plaque assay, and has always tested negative. Stress (such as chemical exposure) could possibly activate the virus.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.03% (w/v) EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:5 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation	Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C