JEG-3(ICGE-JEG-3,IC-5530)
Catalog No.
Size
Price
IC-5530
10^6
$459.00
IC-5530
10^6
�504.90
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue | placenta |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | choriocarcinoma |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Karyotype | This is a hypertriploid human cell line. The modal chromosome number is 71, occurring at 34%, and polyploidy at 2.6%. The t(4;11)(p15;q13), i(13q), t(10p15q), del(18)(q21), and 6 other markers are common to most cells, and two other markers are found in some. Giant satellites are seen in one N14, and two N22. N2, N5, and N9 have 4 copies, and N7, N13, N18, N21 and X a single copy. A single Y chromosome is detected by Q-band examination. |
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| Derivation |
This is one of six clonally derived lines isolated from the Woods strain of the Erwin-Turner tumor by Kohler and associates.
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| Genes Expressed |
human chorionic gonadotropin (hCG), human chorionic somatomammotropin (placental lactogen); progesterone
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| Tumorigenic | Yes |
| Effects |
Yes, in nude mice; forms malignant tumor consistent with choriocarcinoma
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| Comments |
The cells are able to transform steroid precursors to estrone and estradiol.
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Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle''''''''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
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| Cryopreservation |
Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
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| Culture Conditions |
Temperature: 37°C
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