KU812(ICGE-KU812,IC-22397)
Catalog No.
Size
Price
IC-22397
10^6
$529.00
IC-22397
10^6
�581.90
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue | peripheral blood |
| Cell Type | basophil |
| Product Format | frozen |
| Morphology | myeloblast |
| Culture Properties | suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | chronic myelogenous leukemia (CML) |
| Age | 38 years adult |
| Gender | male |
| Ethnicity | Japanese |
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Karyotype | 58, XY, t(9;22)(q34;q11), i(17q), +1, +4, +6, +6, +8, +der(9)t(9;22), + der(9)t(9;22), +der(11)q(11), +14, +15, +19, +21, +21, -9 Ref |
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| Derivation |
The KU812 cell line was established from the peripheral blood of a patient in blast crisis of chronic myelogenous leukemia.
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| Clinical Data |
male
38 years adult
Japanese
|
| Antigen Expression |
CD3; Homo sapiens
alanyl (membrane) aminopeptidase (ANPEP, CD13); Homo sapiens
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| Comments |
The cells contain at least one Ph1 (Philadelphia) chromosome. The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers.
The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers.
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Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. |
|---|---|
| Subculturing |
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 105 viable cells/mL. Do not allow the cell concentration to exceed 3 x 106 cells/mL.
Medium Renewal: every 2 to 3 days
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| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
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