MIA-PACA-2(ICGE-MIA-PACA-2,IC-81385)
Catalog No.
Size
Price
IC-81385
10^6
$498.00
IC-81385
10^6
�547.80
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue | pancreas |
| Cell Type | epithelial cell |
| Product Format | frozen |
| Morphology | attached epithelial with floating rounded cells |
| Culture Properties | adherent, single cells and loosely attached clusters |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | carcinoma |
| Age | 65 years |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Karyotype | This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent. |
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| Derivation |
The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male.
|
| Clinical Data |
65 years
Caucasian
male
|
| Genes Expressed |
human colony stimulating factor, subclass I (CSF-I); plasminogen activator
|
| Cellular Products |
human colony stimulating factor, subclass I (CSF-I); plasminogen activator
|
| Comments |
The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%.
Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1).
The line is sensitive to asparaginase.
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Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco''''''''''''''''s Modified Eagle''''''''''''''''s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%; horse serum to a final concentration of 2.5%. |
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| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
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| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
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| Culture Conditions |
Temperature: 37°C
|
