MLECs,NCTC clone 1469(IC-31916）
Catalog No.
Size
Price
IC-31916
10^6 Cells
$456.00
IC-31916
10^6 Cells
�547.80
Overview
| Organism | Mus musculus, mouse |
|---|---|
| Tissue | liver |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Age | newborn |
| Gender | male |
| Strain | C3H/An |
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Karyotype |
modal number = 41; range = 38 to 86. Two marker chromosomes present: One metacentric with one pair of arms having achromatic gaps near centromere; and an acrocentric with satellites. |
|---|---|
| Antigen Expression |
H-2k
|
| Virus Susceptibility |
Vesicular stomatitis virus Human poliovirus 1 |
| Comments |
Tested and found negative for ectromelia virus (mousepox).
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco''''s Modified Eagle''''s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Subcultures are prepared by scraping. Remove old medium, add fresh medium and dislodge the cells from the floor of the flask. Aspirate cells and dispense the suspension into new flasks. A standard trypsinizaton may be used if desired. The culture medium in heavy cultures may be turbid in appearance and and give the gross impression of bacterial contamination. It is characteristic of this line to shed viable cells into the medium, thus rendering the medium turbid. The shed cells are viable and may be used to initiate new cultures.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
|
| Culture Conditions |
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
|
