Mouse Cell Lines-Cell Lines-InCellGene LLC.-CellLines,Biomedicine reasearch Product,Cell Biology,Cell Culture,293T Cell,Media

Organism	Mus musculus, mouse
Tissue	embryo
Cell Type	fibroblast
Product Format	frozen
Morphology	fibroblast
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age	embryo
Applications	This cell line is a suitable transfection host.
Storage Conditions	liquid nitrogen vapor phase
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation	L1 is a continuous substrain of 3T3 (Swiss albino) developed through clonal isolation.
Receptor Expression	insulin, expressed
Genes Expressed	triglycerides
Cellular Products	triglycerides
Comments	The cells undergo a pre-adipose to adipose like conversion as they progress from a rapidly dividing to a confluent and contact inhibited state. A high serum content in the medium enhances fat accumulation [PubMed ID: 4426090]. Tested and found negative for ectromelia virus (mousepox). This line is also designated as ATCC CCL-92.1. ATCC CL-173 was deposited in 1974 without passage number information from the depositor.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco''s Modified Eagle''s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
Subculturing	Protocol: Never allow culture to become completely confluent. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. The recommended inoculum is 2 to 3 X 103 cells/cm2. Subculture before cultures become 70 to 80% confluent or when cells reach 5 to 6 X10(4) viable cells/cm2. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product. Incubate cultures at 37°C. Interval: Every three days Medium Renewal: 2 to 3 times per week
Cryopreservation	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C Growth Conditions: The serum used is important in culturing this line. Calf serum is recommended and not fetal bovine serum. The calf serum initially employed and found to be satisfactory was from the Colorado Serum Co. Denver.