IEC-6（ICGE-IEC-6，IC-30354）
Catalog No.
Size
Price
IC-30354
10^6
$589.00
IC-30354
10^6
�647.90
Overview
OrganismRattus norvegicus, ratTissue
DiseasenormalGendermaleApplications
small intestine/epithelium
Product FormatfrozenMorphologyepithelialCulture PropertiesadherentBiosafety Level1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
This cell line is a suitable transfection host.
Storage Conditionsliquid nitrogen vapor phaseProperties
Clinical Data
male
Comments
Cell growth is inhibited by cortisol.
The cells express antigens characteristic of intestinal epithelia.
The depositor notes that the cells maintain a constant growth rate and cell morphology until approximately the 20th passage, thereafter, either cell growth rapidly declines or morphological changes are observed.
Background
| Complete Growth Medium |
Dulbecco''s modified Eagle''s medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 90%; fetal bovine serum, 10% |
|---|---|
| Subculturing |
Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week
|
| Cryopreservation |
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
|
| Culture Conditions |
Temperature: 37°C
|
