Human Cell Lines-Cell Lines-InCellGene LLC.-CellLines,Biomedicine reasearch Product,Cell Biology,Cell Culture,293T Cell,Media

OrganismHomo sapiens, humanTissuestomachProduct FormatfrozenMorphologyepithelialCulture PropertiesadherentBiosafety Level2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Diseasegastric adenocarcinomaAge54 yearsGenderfemaleEthnicityCaucasianApplications

This cell line is a suitable transfection host.

Storage Conditionsliquid nitrogen vapor phase

Karyotype	This is a hyperdiploid human cell line. The modal chromosome number was 49, occurring in 60% of cells. The rate of polyploidy was 3.6%. Single copy each for der(8)t(1;8) (q12;p23), der(19)t(19;?) (q13.6;?), minute chromosome M3, and C-group-like M12 was seen in all cells. The origins of both M3 and M12 defied identification presently. The t(13q14q) occurred in some. Generally there were three copies for N20, and single copy for X, N8 and N18. Occasionally there were three copies for N14.

Derivation	The AGS cell line was derived from fragments of a tumor resected from a patient who had received no prior therapy.
Clinical Data	female 54 years Caucasian
Tumorigenic	Yes
Effects	Yes, in athymic BALB/c mice
Comments	The cells have a plating efficiency of 34% in the medium below. The line was cured at the ATCC of a prior mycoplasma infection . Subsequently, AGS has been determined to be infected with Parainfluenza type 5 (PIV5 formerly known as SV5). [PubMed: 17509637]

Complete Growth Medium	The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Temperature: 37°C