RAW264.7(ICGE-RAW264.7,IC-82962)
Catalog No.
Size
Price
IC-82962
10^6
$498.00
IC-82962
10^6
�547.80
Overview
| Organism | Mus musculus, mouse |
|---|---|
| Tissue | Abelson murine leukemia virus-induced tumor; ascites |
| Cell Type | macrophage; Abelson murine leukemia virus transformed |
| Product Format | frozen |
| Morphology | monocyte/macrophage |
| Culture Properties | adherent |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Abelson murine leukemia virus-induced tumor |
| Age | adult |
| Gender | male |
| Strain | BALB/c |
| Applications |
This cell line is a suitable transfection host.
The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets. LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets. |
| Storage Conditions | liquid nitrogen vapor phase |
Properties
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| Derivation |
This line was established from a tumor induced by Abelson murine leukemia virus.
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| Clinical Data |
male adult
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| Antigen Expression |
H-2d
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| Receptor Expression |
Receptor expression: complement (C3) Ref
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| Genes Expressed |
lysozyme, H-2d Tested and found negative for ectromelia virus (mousepox). |
| Cellular Products |
lysozyme
|
| Comments | This cell line is easy to propagate, high efficiency for DNA transfection, sensitivity to RNA interference, and supports replication of murine noroviruses. This cell line is negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2). When this line was established, it was described as not secreting detectable virus particles and negative using the XC plaque formation assay. Based on a published study by Dr. Janet W. Hartley in 2008, this line was demonstrated to express ecotropic and polytropic MuLV, and is positive using the XC plaque assay for virus replication.Ref |
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco''s Modified Eagle''s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Subcultures are prepared by scraping. For a 75 cm2 flask, remove all but 10 mL culture medium (adjust amount accordingly for other culture vessels). Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Dislodge cells from the flask substrate with a cell scraper; aspirate and add appropriate aliquots of the cell suspension into new culture vessels.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Replace or add medium every 2 to 3 days.
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| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
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