Human Cell Lines-Cell Lines-InCellGene LLC.-CellLines,Biomedicine reasearch Product,Cell Biology,Cell Culture,293T Cell,Media

Organism	Homo sapiens, human
Tissue	colon
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Dukes'''' type C, grade III, colorectal adenocarcinoma
Age	81 years
Gender	female
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase

Clinical Data	female 81 years Caucasian
Antigen Expression	blood type O; Rh+
Oncogene	myc +; myb +; ras +; fos +; p53 +; sis -; abl -; ros -; src -
Genes Expressed	carcinoembryonic antigen (CEA) 7 ng/106 cells/10 days; colon specific antigen (CSAp) 750 units in 0.5 mL cell sonicate; keratin
Cellular Products	carcinoembryonic antigen (CEA) 7 ng/10 exp6 cells/10 days; colon specific antigen (CSAp) 750 units in 0.5 ml cell sonicate; keratin
Tumorigenic	Yes
Effects	Yes, in nude mice Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Comments	The cells are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb and fos oncogenes. N-myc and sis oncogene expression were not detected.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Leibovitz''''s L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
Subculturing	Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37°C. Add fresh medium, disperse cells and centrifuge to pellet the cells. Resuspend in fresh medium and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:15 is recommended Medium Renewal: 1 to 2 times per week
Culture Conditions	Atmosphere: air, 100%

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Leibovitz''''s L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)

Subculturing

Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37°C. Add fresh medium, disperse cells and centrifuge to pellet the cells. Resuspend in fresh medium and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:15 is recommended

Medium Renewal: 1 to 2 times per week

Culture Conditions

Atmosphere: air, 100%