THP-1(ICGE-THP-1,IC-85382)
Catalog No.
Size
Price
IC-85382
10^6
$498.00
IC-85382
10^6
�547.80
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue | peripheral blood |
| Cell Type | monocyte |
| Product Format | frozen |
| Morphology | monocyte |
| Culture Properties | suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | acute monocytic leukemia |
| Age | 1 year infant |
| Gender | male |
| Applications |
These cells are suitable as a transfection host.
|
| Storage Conditions | liquid nitrogen vapor phase |
Properties
|
|
|
|---|---|
| Antigen Expression |
HLA A2, A9, B5, DRw1, DRw2
|
| Receptor Expression |
complement (C3), expressed
Fc, expressed
|
| Genes Expressed |
lysozyme,HLA A2, A9, B5, DRw1, DRw2
|
| Cellular Products |
lysozyme
|
| Comments |
The cells are phagocytic (for both latex beads and sensitized erythrocytes) and lack surface and cytoplasmic immunoglobulin.
Monocytic differentiation can be induced with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA).
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2-4 x 105 viable cells/mL. Subculture when cell concentration reaches 8x105 cells/mL.Do not allow the cell concentration to exceed 1 x 106 cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Medium Renewal: Every 2 to 3 days
|
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
|
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
|
